Literature DB >> 7765667

Large scale production and semi-purification of kedarcidin in a 1000-L fermentor.

K S Lam1, D R Gustavson, J M Veitch, S Forenza, J Ross, D Miller, J Roach, W B Lebherz, K Poole.   

Abstract

Actinomycete strain ATCC 53650 was grown in a 1000-L fermentor containing 680 L of medium and the production of kedarcidin was monitored by HPLC. The titers of kedarcidin in the fermentor cultures were 0.49-0.53 mg ml-1. A quick and efficient purification method involving the use of anion exchange resin DE23 (batch adsorption-desorption) and an ultrafiltration system yielded high recovery (65% yield) of kedarcidin from the fermentor culture. Over 200 grams of lyophilized kedarcidin of 70% purity was recovered from each of two 1000-L fermentor cultures using this process.

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Year:  1994        PMID： 7765667     DOI： 10.1007/BF01577219

Source DB:  PubMed          Journal:  J Ind Microbiol        ISSN： 0169-4146


  12 in total

1.  Kedarcidin, a new chromoprotein antitumor antibiotic. II. Isolation, purification and physico-chemical properties.

Authors:  S J Hofstead; J A Matson; A R Malacko; H Marquardt
Journal:  J Antibiot (Tokyo)       Date:  1992-08       Impact factor: 2.649

2.  NEOCARZINOSTATIN, AN ANTITUMOR ANTIBIOTIC OF HIGH MOLECULAR WEIGHT. ISOLATION, PHYSIOCHEMICAL PROPERTIES AND BIOLOGICAL ACTIVITIES.

Authors:  N ISHIDA; K MIYAZAKI; K KUMAGAI; M RIKIMARU
Journal:  J Antibiot (Tokyo)       Date:  1965-03       Impact factor: 2.649

3.  Structure of the antitumor protein neocarzinostatin. Purification, amino acid composition, disulfide reduction, and isolation and composition of tryptic peptides.

Authors:  H Maeda; C B Glaser; J Czombos; J Meienhoffer
Journal:  Arch Biochem Biophys       Date:  1974-10       Impact factor: 4.013

4.  A new antibiotic, macromomycin, exhibiting antitumor and antimicrobial activity.

Authors:  H Chimura; M Ishizuka; M Hamada; S Hori; K Kimura
Journal:  J Antibiot (Tokyo)       Date:  1968-01       Impact factor: 2.649

5.  A new macromolecular antitumor antibiotic, C-1027. I. Discovery, taxonomy of producing organism, fermentation and biological activity.

Authors:  J L Hu; Y C Xue; M Y Xie; R Zhang; T Otani; Y Minami; Y Yamada; T Marunaka
Journal:  J Antibiot (Tokyo)       Date:  1988-11       Impact factor: 2.649

6.  A new macromolecular antitumor antibiotic, C-1027. II. Isolation and physico-chemical properties.

Authors:  T Otani; Y Minami; T Marunaka; R Zhang; M Y Xie
Journal:  J Antibiot (Tokyo)       Date:  1988-11       Impact factor: 2.649

Review 7.  Control of antibiotic biosynthesis.

Authors:  J F Martin; A L Demain
Journal:  Microbiol Rev       Date:  1980-06

8.  Kedarcidin, a new chromoprotein antitumor antibiotic. I. Taxonomy of producing organism, fermentation and biological activity.

Authors:  K S Lam; G A Hesler; D R Gustavson; A R Crosswell; J M Veitch; S Forenza; K Tomita
Journal:  J Antibiot (Tokyo)       Date:  1991-05       Impact factor: 2.649

9.  Selective proteolytic activity of the antitumor agent kedarcidin.

Authors:  N Zein; A M Casazza; T W Doyle; J E Leet; D R Schroeder; W Solomon; S G Nadler
Journal:  Proc Natl Acad Sci U S A       Date:  1993-09-01       Impact factor: 11.205

10.  Primary structure of macromomycin, an antitumor antibiotic protein.

Authors:  T S Samy; K S Hahm; E J Modest; G W Lampman; H T Keutmann; H Umezawa; W C Herlihy; B W Gibson; S A Carr; K Biemann
Journal:  J Biol Chem       Date:  1983-01-10       Impact factor: 5.157
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  1 in total

1.  Controlling instability in gacS-gacA regulatory genes during inoculant production of Pseudomonas fluorescens biocontrol strains.

Authors:  B K Duffy; G Défago
Journal:  Appl Environ Microbiol       Date:  2000-08       Impact factor: 4.792
  1 in total

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