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Literature DB >> 7764901

Engineering proteins to facilitate bioprocessing.

Abstract

Genetic engineering is now being applied to aid the purification of recombinant proteins. The addition of specifically designed tags or the modification of sequences within the target-gene product has enabled the development of novel strategies for downstream processing that can be employed for efficient recovery of both native or modified proteins. This article discusses novel trends in genetic engineering that aid the bioprocessing of recombinant proteins.

Mesh：

Substances：
Recombinant Proteins

Year: 1994 PMID： 7764901 DOI： 10.1016/0167-7799(94)90080-9

Source DB: PubMed Journal: Trends Biotechnol ISSN： 0167-7799 Impact factor: 19.536

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Cited

22 in total

1. Escherichia coli maltose-binding protein is uncommonly effective at promoting the solubility of polypeptides to which it is fused.

Authors: R B Kapust; D S Waugh
Journal: Protein Sci Date: 1999-08 Impact factor: 6.725

2. Combined effect of protein fusion and signal sequence greatly enhances the production of recombinant human GM-CSF in Escherichia coli.

Authors: Palash Bhattacharya; Gaurav Pandey; Poonam Srivastava; Krishna Jyoti Mukherjee
Journal: Mol Biotechnol Date: 2005-06 Impact factor: 2.695

3. RNA affinity tags for the rapid purification and investigation of RNAs and RNA-protein complexes.

Authors: Scott C Walker; Felicia H Scott; Chatchawan Srisawat; David R Engelke
Journal: Methods Mol Biol Date: 2008

4. Purification of a rat neurotensin receptor expressed in Escherichia coli.

Authors: J Tucker; R Grisshammer
Journal: Biochem J Date: 1996-08-01 Impact factor: 3.857

5. Antigenic role of the endosymbionts of filarial nematodes: IgG response against the Wolbachia surface protein in cats infected with Dirofilaria immitis.

Authors: C Bazzocchi; F Ceciliani; J W McCall; I Ricci; C Genchi; C Bandi
Journal: Proc Biol Sci Date: 2000-12-22 Impact factor: 5.349

Engineering proteins to facilitate bioprocessing.

1. Escherichia coli maltose-binding protein is uncommonly effective at promoting the solubility of polypeptides to which it is fused.

2. Combined effect of protein fusion and signal sequence greatly enhances the production of recombinant human GM-CSF in Escherichia coli.

3. RNA affinity tags for the rapid purification and investigation of RNAs and RNA-protein complexes.

4. Purification of a rat neurotensin receptor expressed in Escherichia coli.

5. Antigenic role of the endosymbionts of filarial nematodes: IgG response against the Wolbachia surface protein in cats infected with Dirofilaria immitis.

6. A family of LIC vectors for high-throughput cloning and purification of proteins.

Review 7. Strategies for achieving high-level expression of genes in Escherichia coli.

8. Biotinylated-sortase self-cleavage purification (BISOP) method for cell-free produced proteins.

9. Molecular beacon aptamers for direct and universal quantitation of recombinant proteins from cell lysates.

Review 10. Toxins from cone snails: properties, applications and biotechnological production.