Protein engineering for unusual environments.

The ability to use proteins in unusual or non-natural environments greatly expands their potential applications in biotechnology. Because natural selection has neither maximized the stability of proteins nor optimized them to function under unusual conditions, there is considerable room for their improvement by protein engineering. Significant advances reported within the past year include a dramatic demonstration of a protein's ability to tolerate changes in its amino acid sequence, large increases in protein stability, and the use of random mutagenesis to obtain novel enzymatic properties. Approaches using random or site-directed mutagenesis have been successful in generating proteins able to function in an extended range of environments.