Literature DB >> 7758943

Regulation of genomic imprinting by gametic and embryonic processes.

J R Chaillet1, D S Bader, P Leder.   

Abstract

Parental genomic imprinting refers to the phenomenon by which alleles behave differently depending on the sex of the parent from which they are inherited. In the case of the murine transgene RSVIgmyc, imprinting is manifest in two ways: differential DNA methylation and differential expression. In inbred FVB/N mice, a transgene inherited from a male parent is undermethylated and expressed; a transgene inherited from the female parent is overmethylated and silent. Using a series of RSVIgmyc constructs and transgenic mice, we show that the imprinting of this transgene requires a cis-acting signal that is principally derived from the repeat sequences that make up the 3' portion of the murine immunoglobulin alpha heavy-chain switch region. Such imprinting is relatively independent of the site of transgene insertion but is influenced by the structure of the transgene itself. Imprinting is also modulated by genetic background. Detailed studies indicate that the paternal allele is undermethylated and expressed in inbred FVB/N mice and in heterozygous F1 FVB/N/C57Bl/6J mice but is overmethylated and silent in inbred C57Bl/6J mice. Consequently, the FVB/N genome appears to carry alleles of modulating genes that dominantly block methylation and permit expression of the paternally imprinted transgene. Furthermore, our results suggest that overmethylation is the default status of both parental alleles and that the paternal allele can be marked in trans by polymorphic factors that act in postblastocyst embryos.

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Year:  1995        PMID: 7758943     DOI: 10.1101/gad.9.10.1177

Source DB:  PubMed          Journal:  Genes Dev        ISSN: 0890-9369            Impact factor:   11.361


  19 in total

1.  Genetic conflicts in genomic imprinting.

Authors:  A Burt; R Trivers
Journal:  Proc Biol Sci       Date:  1998-12-22       Impact factor: 5.349

2.  Phenotypic variation in a genetically identical population of mice.

Authors:  K Weichman; J R Chaillet
Journal:  Mol Cell Biol       Date:  1997-09       Impact factor: 4.272

3.  Specific differentially methylated domain sequences direct the maintenance of methylation at imprinted genes.

Authors:  Bonnie Reinhart; Ariane Paoloni-Giacobino; J Richard Chaillet
Journal:  Mol Cell Biol       Date:  2006-09-05       Impact factor: 4.272

4.  Gtl2lacZ, an insertional mutation on mouse chromosome 12 with parental origin-dependent phenotype.

Authors:  K Schuster-Gossler; D Simon-Chazottes; J L Guenet; J Zachgo; A Gossler
Journal:  Mamm Genome       Date:  1996-01       Impact factor: 2.957

Review 5.  Genomic imprinting in mammals.

Authors:  Denise P Barlow; Marisa S Bartolomei
Journal:  Cold Spring Harb Perspect Biol       Date:  2014-02-01       Impact factor: 10.005

6.  Shared role for differentially methylated domains of imprinted genes.

Authors:  Bonnie Reinhart; Mariam Eljanne; J Richard Chaillet
Journal:  Mol Cell Biol       Date:  2002-04       Impact factor: 4.272

7.  A transgene insertion creating a heritable chromosome deletion mouse model of Prader-Willi and angelman syndromes.

Authors:  J M Gabriel; M Merchant; T Ohta; Y Ji; R G Caldwell; M J Ramsey; J D Tucker; R Longnecker; R D Nicholls
Journal:  Proc Natl Acad Sci U S A       Date:  1999-08-03       Impact factor: 11.205

8.  A cis-acting element that directs the activity of the murine methylation modifier locus Ssm1.

Authors:  P Engler; L T Doglio; G Bozek; U Storb
Journal:  Proc Natl Acad Sci U S A       Date:  1998-09-01       Impact factor: 11.205

9.  Role of the inositol polyphosphate-4-phosphatase type II Inpp4b in the generation of ovarian teratomas.

Authors:  Ashwini Balakrishnan; J Richard Chaillet
Journal:  Dev Biol       Date:  2012-10-16       Impact factor: 3.582

10.  Ovarian teratomas associated with the insertion of an imprinted transgene.

Authors:  M K Fafalios; E A Olander; M F Melhem; J R Chaillet
Journal:  Mamm Genome       Date:  1996-03       Impact factor: 2.957

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