Literature DB >> 7751376

Recombinant 46-kilodalton surface antigen (P46) of Mycoplasma hyopneumoniae expressed in Escherichia coli can be used for early specific diagnosis of mycoplasmal pneumonia of swine by enzyme-linked immunosorbent assay.

S Futo1, Y Seto, M Okada, S Sato, T Suzuki, K Kawai, Y Imada, Y Mori.   

Abstract

The 46-kDa surface antigen (P46) is the early and species-specific immunogenic protein of Mycoplasma hyopneumoniae. Three TGA codons encoding tryptophan in the P46 gene were replaced with TGG by an in vitro mutagenesis technique. The mutated P46 gene was expressed in Escherichia coli by using the chelating peptide tag system. The purified recombinant P46 was successfully used in an enzyme-linked immunosorbent assay for detection of antibodies against M. hyopneumoniae in swine serum. It did not cross-react with sera from swine infected with Mycoplasma flocculate, Mycoplasma hyorhinis, or Mycoplasma hyosynoviae. With this method, mycoplasmal pneumonia of swine was detectable within 2 weeks after infection.

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Year:  1995        PMID： 7751376      PMCID： PMC228013          DOI： 10.1128/jcm.33.3.680-683.1995

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  18 in total

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Journal:  Methods Enzymol       Date:  1987       Impact factor: 1.600

5.  Serological cross-reactivity of porcine reference antisera to Mycoplasma hyopneumoniae, M. flocculare, M. hyorhinis and M. hyosynoviae indicated by the enzyme-linked immunosorbent assay, complement fixation and indirect hemagglutination tests.

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Journal:  Can J Comp Med       Date:  1984-04

Review 6.  Post-translational modification and processing of outer membrane prolipoproteins in Escherichia coli.

Authors:  S Mizushima
Journal:  Mol Cell Biochem       Date:  1984       Impact factor: 3.396

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Authors:  Y Mori; Y Yoshida; C Kuniyasu; K Hashimoto
Journal:  Natl Inst Anim Health Q (Tokyo)       Date:  1983

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Authors:  B K Smiley; F C Minion
Journal:  Gene       Date:  1993-11-30       Impact factor: 3.688

9.  Use of monoclonal antibody in an enzyme-linked immunosorbent assay (ELISA) for the detection of antibodies against Mycoplasma hyopneumoniae.

Authors:  Y Mori; T Hamaoka; S Sato
Journal:  Isr J Med Sci       Date:  1987-06

10.  UGA is read as tryptophan in Mycoplasma capricolum.

Authors:  F Yamao; A Muto; Y Kawauchi; M Iwami; S Iwagami; Y Azumi; S Osawa
Journal:  Proc Natl Acad Sci U S A       Date:  1985-04       Impact factor: 11.205
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  8 in total

1.  Detection of Mycoplasma hyopneumoniae by air sampling with a nested PCR assay.

Authors:  K D Stärk; J Nicolet; J Frey
Journal:  Appl Environ Microbiol       Date:  1998-02       Impact factor: 4.792

2.  Species-specific monoclonal antibodies to Escherichia coli-expressed p36 cytosolic protein of Mycoplasma hyopneumoniae.

Authors:  J Caron; N Sawyer; B Ben Abdel Moumen; K Cheikh Saad Bouh; S Dea
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3.  Immunostimulatory effects of recombinant Erysipelothrix rhusiopathiae expressing porcine interleukin-18 in mice and pigs.

Authors:  Yohsuke Ogawa; Yu Minagawa; Fang Shi; Masahiro Eguchi; Yoshihiro Muneta; Yoshihiro Shimoji
Journal:  Clin Vaccine Immunol       Date:  2012-07-03

4.  Heterologous Expression, Purification, and Immunomodulatory Effects of Recombinant Lipoprotein GUDIV-103 Isolated from Ureaplasma diversum.

Authors:  Manoel Neres Santos-Junior; Wanderson Souza Neves; Ronaldo Silva Santos; Palloma Porto Almeida; Janaina Marinho Fernandes; Bruna Carolina de Brito Guimarães; Maysa Santos Barbosa; Lucas Santana Coelho da Silva; Camila Pacheco Gomes; Beatriz Almeida Sampaio; Izadora de Souza Rezende; Thiago Macedo Lopes Correia; Nayara Silva de Macedo Neres; Guilherme Barreto Campos; Bruno Lopes Bastos; Jorge Timenetsky; Lucas Miranda Marques
Journal:  Microorganisms       Date:  2022-05-16

5.  Monoclonal antibodies to Escherichia coli-expressed P46 and P65 membranous proteins for specific immunodetection of Mycoplasma hyopneumoniae in lungs of infected pigs.

Authors:  K Cheikh Saad Bouh; F Shareck; S Dea
Journal:  Clin Diagn Lab Immunol       Date:  2003-05

6.  Invasion of melanoma cells by Mycoplasma hyorhinis: enhancement by protease treatment.

Authors:  Jonathan D Kornspan; Mark Tarshis; Shlomo Rottem
Journal:  Infect Immun       Date:  2009-11-16       Impact factor: 3.441

7.  Comparative genomic analyses of Mycoplasma hyopneumoniae pathogenic 168 strain and its high-passaged attenuated strain.

Authors:  Wei Liu; Shaobo Xiao; Mao Li; Shaohua Guo; Sha Li; Rui Luo; Zhixin Feng; Bin Li; Zhemin Zhou; Guoqing Shao; Huanchun Chen; Liurong Fang
Journal:  BMC Genomics       Date:  2013-02-05       Impact factor: 3.969

8.  Development of an ELISA using a recombinant P46-like lipoprotein for diagnosis of Mycoplasma pulmonis infection in rodents.

Authors:  Atsushi Asano; Daisuke Torigoe; Nobuya Sasaki; Takashi Agui
Journal:  J Vet Med Sci       Date:  2013-09-20       Impact factor: 1.267
  8 in total

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