Literature DB >> 6166972

Tween 20 soluble proteins of Mycoplasma hyopneumoniae as antigen for an enzyme linked immunosorbent assay.

J Nicolet, P Paroz, S Bruggmann.   

Abstract

The preparation of an antigen of Mycoplasma hyopneumoniae containing selected proteins after treatment with the neutral detergent Tween 20 and purification on Sephadex G25 is described. The antigenic activity and the specificity were determined by means of an enzyme linked immunosorbent assay and compared with that of a whole-cell, SDS-solubilised antigen. The Tween 20 purified antigen showed a comparable activity but a higher specificity.

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Year:  1980        PMID： 6166972

Source DB:  PubMed          Journal:  Res Vet Sci        ISSN： 0034-5288            Impact factor:   2.534


  9 in total

1.  Mycoplasma hyopneumoniae colonization of pigs sired by different boars.

Authors:  Alvaro Ruiz; Lucina Galina; Carlos Pijoan
Journal:  Can J Vet Res       Date:  2002-04       Impact factor: 1.310

2.  Preparation of Mycoplasma hyopneumoniae antigen for the enzyme-linked immunosorbent assay.

Authors:  S Kazama; T Yagihashi; K Seto
Journal:  Can J Vet Res       Date:  1989-04       Impact factor: 1.310

3.  Detection of Mycoplasma hyopneumoniae by air sampling with a nested PCR assay.

Authors:  K D Stärk; J Nicolet; J Frey
Journal:  Appl Environ Microbiol       Date:  1998-02       Impact factor: 4.792

4.  Stimulation of immunoglobulin-containing cells and isotype-specific antibody response in experimental Mycoplasma hyopneumoniae infection in specific-pathogen-free pigs.

Authors:  M Suter; M Kobisch; J Nicolet
Journal:  Infect Immun       Date:  1985-09       Impact factor: 3.441

5.  Erysipelothrix rhusiopathiae YS-1 as a live vaccine vehicle for heterologous protein expression and intranasal immunization of pigs.

Authors:  Yoshihiro Shimoji; Eiji Oishi; Takashi Kitajima; Yoshihiro Muneta; Shinya Shimizu; Yasuyuki Mori
Journal:  Infect Immun       Date:  2002-01       Impact factor: 3.441

6.  Recombinant 46-kilodalton surface antigen (P46) of Mycoplasma hyopneumoniae expressed in Escherichia coli can be used for early specific diagnosis of mycoplasmal pneumonia of swine by enzyme-linked immunosorbent assay.

Authors:  S Futo; Y Seto; M Okada; S Sato; T Suzuki; K Kawai; Y Imada; Y Mori
Journal:  J Clin Microbiol       Date:  1995-03       Impact factor: 5.948

7.  Diagnostic specificity, sensitivity and cross-reactivity of an enzyme-linked immunosorbent assay for the detection of antibody against Leptospira interrogans serovars pomona, sejroe and hardjo in cattle.

Authors:  H J Cho; S P Gale; S A Masri; K L Malkin
Journal:  Can J Vet Res       Date:  1989-07       Impact factor: 1.310

8.  Measurement of antibody to Ureaplasma urealyticum by an enzyme-linked immunosorbent assay and detection of antibody responses in patients with nongonococcal urethritis.

Authors:  M B Brown; G H Cassell; D Taylor-Robinson; M C Shepard
Journal:  J Clin Microbiol       Date:  1983-02       Impact factor: 5.948

9.  Evaluation of enzyme-linked immunosorbent assays for detection of Mycoplasma bovis-specific antibody in bison sera.

Authors:  Karen B Register; Randy E Sacco; Steven C Olsen
Journal:  Clin Vaccine Immunol       Date:  2013-07-10
  9 in total

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