Literature DB >> 7751368

Inactivation of polymerase inhibitors for Mycobacterium tuberculosis DNA amplification in sputum by using capture resin.

M Amicosante1, L Richeldi, G Trenti, G Paone, M Campa, A Bisetti, C Saltini.   

Abstract

Endogenous PCR inhibitors hamper DNA amplification of Mycobacterium tuberculosis DNA in sputum samples. Use of an anion binding resin (GeneReleaser [GR]) improved PCR sensitivity from 77% (27 of 35 culture-positive samples) with phenol-chloroform extraction (P-C) alone to 91% (GR without P-C; P > 0.05) and 100% (P-C and GR combined; P < 0.05).

Entities:  

Mesh：

Substances：

Year:  1995        PMID： 7751368      PMCID： PMC228003          DOI： 10.1128/jcm.33.3.629-630.1995

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  10 in total

1.  Rapid detection of tuberculous and non-tuberculous mycobacteria by polymerase chain reaction amplification of a 162 bp DNA fragment from antigen 85.

Authors:  M Fauville-Dufaux; B Vanfleteren; L De Wit; J P Vincke; J P Van Vooren; M D Yates; E Serruys; J Content
Journal:  Eur J Clin Microbiol Infect Dis       Date:  1992-09       Impact factor: 3.267

2.  Polymerase chain reaction amplification of a repetitive DNA sequence specific for Mycobacterium tuberculosis.

Authors:  K D Eisenach; M D Cave; J H Bates; J T Crawford
Journal:  J Infect Dis       Date:  1990-05       Impact factor: 5.226

3.  DNA enzyme immunoassay: general method for detecting products of polymerase chain reaction.

Authors:  G Mantero; A Zonaro; A Albertini; P Bertolo; D Primi
Journal:  Clin Chem       Date:  1991-03       Impact factor: 8.327

4.  Detection and identification of mycobacteria by amplification of mycobacterial DNA.

Authors:  A J Hance; B Grandchamp; V Lévy-Frébault; D Lecossier; J Rauzier; D Bocart; B Gicquel
Journal:  Mol Microbiol       Date:  1989-07       Impact factor: 3.501

5.  Characterization of a Mycobacterium tuberculosis insertion sequence, IS6110, and its application in diagnosis.

Authors:  D Thierry; A Brisson-Noël; V Vincent-Lévy-Frébault; S Nguyen; J L Guesdon; B Gicquel
Journal:  J Clin Microbiol       Date:  1990-12       Impact factor: 5.948

6.  Direct detection of Mycobacterium tuberculosis in sputum by polymerase chain reaction and DNA hybridization.

Authors:  F S Nolte; B Metchock; J E McGowan; A Edwards; O Okwumabua; C Thurmond; P S Mitchell; B Plikaytis; T Shinnick
Journal:  J Clin Microbiol       Date:  1993-07       Impact factor: 5.948

7.  Rapid discrimination of Mycobacterium tuberculosis strains by random amplified polymorphic DNA analysis.

Authors:  C J Linton; H Jalal; J P Leeming; M R Millar
Journal:  J Clin Microbiol       Date:  1994-09       Impact factor: 5.948

8.  Large-scale use of polymerase chain reaction for detection of Mycobacterium tuberculosis in a routine mycobacteriology laboratory.

Authors:  J E Clarridge; R M Shawar; T M Shinnick; B B Plikaytis
Journal:  J Clin Microbiol       Date:  1993-08       Impact factor: 5.948

9.  Detection and identification of mycobacteria by amplification of a segment of the gene coding for the 32-kilodalton protein.

Authors:  H Soini; M Skurnik; K Liippo; E Tala; M K Viljanen
Journal:  J Clin Microbiol       Date:  1992-08       Impact factor: 5.948

10.  Comparison of polymerase chain reaction amplification of two mycobacterial DNA sequences, IS6110 and the 65kDa antigen gene, in the diagnosis of tuberculosis.

Authors:  D A Walker; I K Taylor; D M Mitchell; R J Shaw
Journal:  Thorax       Date:  1992-09       Impact factor: 9.139
  10 in total
  25 in total

1.  Simultaneous detection of Bacteroides forsythus and Prevotella intermedia by 16S rRNA gene-directed multiplex PCR.

Authors:  G Conrads; T F Flemmig; I Seyfarth; F Lampert; R Lütticken
Journal:  J Clin Microbiol       Date:  1999-05       Impact factor: 5.948

2.  Rapid identification of mycobacteria and drug-resistant Mycobacterium tuberculosis by use of a single multiplex PCR and DNA sequencing.

Authors:  Ailyn C Pérez-Osorio; David S Boyle; Zachary K Ingham; Alla Ostash; Romesh K Gautom; Craig Colombel; Yolanda Houze; Brandon T Leader
Journal:  J Clin Microbiol       Date:  2011-12-07       Impact factor: 5.948

3.  Novel multipurpose methodology for detection of mycobacteria in pulmonary and extrapulmonary specimens by smear microscopy, culture, and PCR.

Authors:  Soumitesh Chakravorty; Jaya Sivaswami Tyagi
Journal:  J Clin Microbiol       Date:  2005-06       Impact factor: 5.948

Review 4.  Inhibition and facilitation of nucleic acid amplification.

Authors:  I G Wilson
Journal:  Appl Environ Microbiol       Date:  1997-10       Impact factor: 4.792

5.  Single-tube nested PCR in the diagnosis of tuberculosis.

Authors:  C M Chan; K Y Yuen; K S Chan; W C Yam; K H Yim; W F Ng; M H Ng
Journal:  J Clin Pathol       Date:  1996-04       Impact factor: 3.411

Review 6.  Relevance of nucleic acid amplification techniques for diagnosis of respiratory tract infections in the clinical laboratory.

Authors:  M Ieven; H Goossens
Journal:  Clin Microbiol Rev       Date:  1997-04       Impact factor: 26.132

7.  Advances in Molecular Diagnosis of Tuberculosis.

Authors:  V M Katoch
Journal:  Med J Armed Forces India       Date:  2011-07-21

8.  Detection of CMV DNA in bone marrow transplant recipients: plasma versus leucocyte polymerase chain reaction.

Authors:  P C Woo; S K Lo; K Y Yuen; J S Peiris; H Siau; E K Chiu; R H Liang; T K Chan
Journal:  J Clin Pathol       Date:  1997-03       Impact factor: 3.411

9.  Substances interfering with direct detection of Mycobacterium tuberculosis in clinical specimens by PCR: effects of bovine serum albumin.

Authors:  B A Forbes; K E Hicks
Journal:  J Clin Microbiol       Date:  1996-09       Impact factor: 5.948

10.  Assessment by meta-analysis of PCR for diagnosis of smear-negative pulmonary tuberculosis.

Authors:  Olga L Sarmiento; Kristen A Weigle; Janet Alexander; David J Weber; William C Miller
Journal:  J Clin Microbiol       Date:  2003-07       Impact factor: 5.948
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.