| Literature DB >> 7750203 |
E A Price1, D R Coombe, J C Murray.
Abstract
A static adhesion assay employing 6-carboxy-3',6'-diacetylfluorescein (6-CFDA) as a fluorescent marker has been developed to study the interactions of tumour cell lines with endothelial monolayers. This assay allows simple, safe quantification of cell-cell adhesion using living cells. It has been used to demonstrate that the integrin adhesion molecule VLA-4 mediates the attachment of RPMI-7951 melanoma cells to human umbilical vein endothelial cells (HUVEC) which have been activated by TNF alpha. In addition, MDA-MB-231 breast adenocarcinoma cells display greater adhesion to microvessel endothelial cells than to large vessel endothelial cells.Entities:
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Year: 1995 PMID： 7750203 DOI： 10.1007/BF00132203
Source DB: PubMed Journal: Clin Exp Metastasis ISSN： 0262-0898 Impact factor: 5.150