Literature DB >> 7730292

High-level expression of soluble recombinant RNase P protein from Escherichia coli.

R Rivera-León1, C J Green, B S Vold.   

Abstract

We have expressed recombinant RNase P protein from Escherichia coli in high yield. A hexahistidine sequence at the amino terminus allowed protein purification in a single step. Mass spectrometry confirmed the molecular weight of the purified protein and indicated a purity of > 95%. Protein functionality was demonstrated by reconstitution of active holoenzyme.

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Year:  1995        PMID： 7730292      PMCID： PMC176919          DOI： 10.1128/jb.177.9.2564-2566.1995

Source DB:  PubMed          Journal:  J Bacteriol        ISSN： 0021-9193            Impact factor:   3.490


  21 in total

Review 1.  The varieties of ribonuclease P.

Authors:  S C Darr; J W Brown; N R Pace
Journal:  Trends Biochem Sci       Date:  1992-05       Impact factor: 13.807

2.  Preparation of clinical grade proteins produced by recombinant DNA technologies.

Authors:  B J Takacs; M F Girard
Journal:  J Immunol Methods       Date:  1991-10-25       Impact factor: 2.303

3.  A cluster of nine tRNA genes between ribosomal gene operons in Bacillus subtilis.

Authors:  C J Green; B S Vold
Journal:  J Bacteriol       Date:  1992-05       Impact factor: 3.490

4.  Three-dimensional working model of M1 RNA, the catalytic RNA subunit of ribonuclease P from Escherichia coli.

Authors:  E Westhof; S Altman
Journal:  Proc Natl Acad Sci U S A       Date:  1994-05-24       Impact factor: 11.205

5.  Protein-RNA interactions in the RNase P holoenzyme from Escherichia coli.

Authors:  A Vioque; J Arnez; S Altman
Journal:  J Mol Biol       Date:  1988-08-20       Impact factor: 5.469

6.  Ionic conditions for the cleavage of the tRNA-like structure of turnip yellow mosaic virus by the catalytic RNA of RNase P.

Authors:  C J Green; B S Vold; M D Morch; R L Joshi; A L Haenni
Journal:  J Biol Chem       Date:  1988-08-25       Impact factor: 5.157

7.  Gel retardation analysis of the interaction between C5 protein and M1 RNA in the formation of the ribonuclease P holoenzyme from Escherichia coli.

Authors:  S J Talbot; S Altman
Journal:  Biochemistry       Date:  1994-02-15       Impact factor: 3.162

8.  Expression, purification, biochemical characterization and inhibition of recombinant Plasmodium falciparum aldolase.

Authors:  H Döbeli; A Trzeciak; D Gillessen; H Matile; I K Srivastava; L H Perrin; P E Jakob; U Certa
Journal:  Mol Biochem Parasitol       Date:  1990-06       Impact factor: 1.759

9.  Rapid and efficient purification of native histidine-tagged protein expressed by recombinant vaccinia virus.

Authors:  R Janknecht; G de Martynoff; J Lou; R A Hipskind; A Nordheim; H G Stunnenberg
Journal:  Proc Natl Acad Sci U S A       Date:  1991-10-15       Impact factor: 11.205

10.  Use of photoaffinity crosslinking and molecular modeling to analyze the global architecture of ribonuclease P RNA.

Authors:  M E Harris; J M Nolan; A Malhotra; J W Brown; S C Harvey; N R Pace
Journal:  EMBO J       Date:  1994-09-01       Impact factor: 11.598
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  9 in total

1.  Bacterial ribonuclease P holoenzyme crosslinking analysis reveals protein interaction sites on the RNA subunit.

Authors:  S M Sharkady; J M Nolan
Journal:  Nucleic Acids Res       Date:  2001-09-15       Impact factor: 16.971

2.  Ionic interactions between PRNA and P protein in Bacillus subtilis RNase P characterized using a magnetocapture-based assay.

Authors:  Jeremy J Day-Storms; S Niranjanakumari; Carol A Fierke
Journal:  RNA       Date:  2004-08-30       Impact factor: 4.942

3.  Active site constraints in the hydrolysis reaction catalyzed by bacterial RNase P: analysis of precursor tRNAs with a single 3'-S-phosphorothiolate internucleotide linkage.

Authors:  J M Warnecke; E J Sontheimer; J A Piccirilli; R K Hartmann
Journal:  Nucleic Acids Res       Date:  2000-02-01       Impact factor: 16.971

4.  The catalytic core of RNase P.

Authors:  C J Green; R Rivera-León; B S Vold
Journal:  Nucleic Acids Res       Date:  1996-04-15       Impact factor: 16.971

5.  The precursor tRNA 3'-CCA interaction with Escherichia coli RNase P RNA is essential for catalysis by RNase P in vivo.

Authors:  Barbara Wegscheid; Roland K Hartmann
Journal:  RNA       Date:  2006-10-24       Impact factor: 4.942

6.  Thermostable RNase P RNAs lacking P18 identified in the Aquificales.

Authors:  Michal Marszalkowski; Jan-Hendrik Teune; Gerhard Steger; Roland K Hartmann; Dagmar K Willkomm
Journal:  RNA       Date:  2006-09-27       Impact factor: 4.942

7.  Probing the architecture of the B. subtilis RNase P holoenzyme active site by cross-linking and affinity cleavage.

Authors:  Somashekarappa Niranjanakumari; Jeremy J Day-Storms; Mahiuddin Ahmed; John Hsieh; Nathan H Zahler; Ronald A Venters; Carol A Fierke
Journal:  RNA       Date:  2007-02-13       Impact factor: 4.942

8.  Expression, purification and characterization of the recombinant ribonuclease P protein component from Bacillus subtilis.

Authors:  S Niranjanakumari; J C Kurz; C A Fierke
Journal:  Nucleic Acids Res       Date:  1998-07-01       Impact factor: 16.971

9.  Nuclear RNase P of Trypanosoma brucei: a single protein in place of the multicomponent RNA-protein complex.

Authors:  Andreas Taschner; Christoph Weber; Aurélie Buzet; Roland K Hartmann; Andreas Hartig; Walter Rossmanith
Journal:  Cell Rep       Date:  2012-06-28       Impact factor: 9.423
  9 in total

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