Literature DB >> 772416

Dependence of transfection efficiency of calcium treated Escherichia coli cells on bacterial genotype and form of Lambda DNA.

L E Drabkina, L V Konevega, O K Legina, M I Mosevitsky.   

Abstract

The transfecting activity of linear lambda DNA is 100 times higher in calcium treated E. coli K12 (lambda i434) than in non-lysogenic strains: the levels of transfection are 1-2.10(7) and 1-2.10(5) infective centers per 1 mug of lambda DNA respectively. The high efficiency of lysogenic cells transfection is not due to the spontaneously liberated "helper" phage. Evidently, it is called forth by transfecting DNA-prophage recombination or/and by inhibition of nuclease activity in lysogenic cells. Both ring forms lambda DNA (supercoiled and open circles) show very low infectivity, if any, in calcinated cells.

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Year:  1976        PMID： 772416     DOI： 10.1007/bf00277309

Source DB:  PubMed          Journal:  Mol Gen Genet        ISSN： 0026-8925


  8 in total

1.  NOVEL INTRA-CELLULAR FORMS OF LAMBDA DNA.

Authors:  E T YOUNG; R L SINSHEIMER
Journal:  J Mol Biol       Date:  1964-12       Impact factor: 5.469

2.  COHESION OF DNA MOLECULES ISOLATED FROM PHAGE LAMBDA.

Authors:  A D Hershey; E Burgi; L Ingraham
Journal:  Proc Natl Acad Sci U S A       Date:  1963-05       Impact factor: 11.205

3.  Genetic transformation in E. coli: the inhibitory role of the recBC DNase.

Authors:  W Wackernagel
Journal:  Biochem Biophys Res Commun       Date:  1973-03-17       Impact factor: 3.575

4.  Calcium-dependent bacteriophage DNA infection.

Authors:  M Mandel; A Higa
Journal:  J Mol Biol       Date:  1970-10-14       Impact factor: 5.469

5.  The role of exonuclease and beta protein of phage lambda in genetic recombination. II. Substrate specificity and the mode of action of lambda exonuclease.

Authors:  D M Carter; C M Radding
Journal:  J Biol Chem       Date:  1971-04-25       Impact factor: 5.157

6.  Influence of proflavine on the cyclization and aggregation of phage lambda DNA.

Authors:  L E Drabkina; L V Konevega; O K Legina; M I Mosevitskii
Journal:  Mol Biol       Date:  1972 Sep-Oct       Impact factor: 1.374

7.  Vegetative bacteriophage lambda-DNA. I. Infectivity in a spheroplast assay.

Authors:  E T Young; R L Sinsheimer
Journal:  J Mol Biol       Date:  1967-11-28       Impact factor: 5.469

8.  Changes in the structure and activity of lambda DNA in a superinfected immune bacterium.

Authors:  V C Bode; A D Kaiser
Journal:  J Mol Biol       Date:  1965-12       Impact factor: 5.469
  8 in total
  1 in total

1.  Packaging recombinant DNA molecules into bacteriophage particles in vitro.

Authors:  B Hohn; K Murray
Journal:  Proc Natl Acad Sci U S A       Date:  1977-08       Impact factor: 11.205
  1 in total

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