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Literature DB >> 7710322

Tetrameric structure and cellular location of catechol 2,3-dioxygenase.

J Winkler¹, L D Eltis, D F Dwyer, M Rohde.

Abstract

Catechol 2,3-dioxygenase from the meta-cleavage pathway encoded on the TOL plasmid of Pseudomonas putida (pWWO) was investigated by electron microscopy. Negatively stained samples of the purified catechol 2,3-dioxygenase revealed that the enzyme consists of four subunits arranged in a tetrahedral conformation. Monoclonal antibodies raised against catechol 2,3-dioxygenase showed highly specific reactions and were used to localize the enzyme in Escherichia coli (pAW31) and P. putida (pWWO), using the protein A-gold technique carried out as a post-embedding immunoelectron microscopy procedure. Our in situ labeling studies revealed a cytoplasmic location of the catechol 2,3-dioxygenase in both cell types.

Entities: Chemical Species

Mesh：

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Year: 1995 PMID： 7710322 DOI： 10.1007/bf00262205

Source DB: PubMed Journal: Arch Microbiol ISSN： 0302-8933 Impact factor: 2.552

19 in total

1. METAPYROCATECHASE. I. PURIFICATION, CRYSTALLIZATION AND SOME PROPERTIES.

Authors: M NOZAKI; H KAGAMIYAMA; O HAYAISHI
Journal: Biochem Z Date: 1963

2. Evaluation of aquatic sediment microcosms and their use in assessing possible effects of introduced microorganisms on ecosystem parameters.

Authors: I Wagner-Döbler; R Pipke; K N Timmis; D F Dwyer
Journal: Appl Environ Microbiol Date: 1992-04 Impact factor: 4.792

3. Isolation of pure IgG1, IgG2a and IgG2b immunoglobulins from mouse serum using protein A-sepharose.

Authors: P L Ey; S J Prowse; C R Jenkin
Journal: Immunochemistry Date: 1978-07

Review 4. Oxygenases and dioxygenases.

Authors: M Nozaki
Journal: Top Curr Chem Date: 1979

Tetrameric structure and cellular location of catechol 2,3-dioxygenase.

1. METAPYROCATECHASE. I. PURIFICATION, CRYSTALLIZATION AND SOME PROPERTIES.

2. Evaluation of aquatic sediment microcosms and their use in assessing possible effects of introduced microorganisms on ecosystem parameters.

3. Isolation of pure IgG1, IgG2a and IgG2b immunoglobulins from mouse serum using protein A-sepharose.

Review 4. Oxygenases and dioxygenases.

5. Regulation of glutamine synthetase. XII. Electron microscopy of the enzyme from Escherichia coli.

6. Metapyrocatechase. II. The role of iron and sulfhydryl groups.

7. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

8. Enhancement of structural preservation and immunocytochemical staining in low temperature embedded pancreatic tissue.

9. Purification, subunit structure, and partial amino acid sequence of metapyrocatechase.

Review 10. Functional and evolutionary relationships among diverse oxygenases.

1. Crystal Structures of L-DOPA Dioxygenase from Streptomyces sclerotialus.