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Literature DB >> 770435

Isolation and characterization of the specialized transducing bacteriophages phi80dargF and lambdah80cI857 dargF: specific cleavage of arginine transducing deoxyribonucleic acid by the endonucleases EcoRI and SmaR.

P M James, D Sens, W Natter, S K Moore, E James.

Abstract

The directed transposition of argF to the tonB locus of the Escherichia coli chromosome and the subsequent isolation of the specialized transducing phage phi80dargF is described. The structure of this phage has been has been determined. A hybrid lambdah80cI857dargF phage has been constructed. Deoxyribonucleic acid isolated from these and their parent bacteriophages has been specifically cleaved by the endonucleases EcoRI and SmaR; the unique deoxyribonucleic acid fragments thus obtained have been resolved and analyzed by electrophoresis in agarose gel.

Entities: Chemical Species

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Year: 1976 PMID： 770435 PMCID： PMC233305 DOI： 10.1128/jb.126.1.487-500.1976

Source DB: PubMed Journal: J Bacteriol ISSN： 0021-9193 Impact factor: 3.490

33 in total

2. Transcription of the argF and argI genes of the arginine biosynthetic regulon of Escherichia coli K12, performed in vitro.

Authors: D Sens; W Natter; R T Garvin; E James
Journal: Mol Gen Genet Date: 1977-09-21

Isolation and characterization of the specialized transducing bacteriophages phi80dargF and lambdah80cI857 dargF: specific cleavage of arginine transducing deoxyribonucleic acid by the endonucleases EcoRI and SmaR.

1. Transduction of linked genetic characters of the host by bacteriophage P1.

2. THE EFFECT OF ACRIDINE DYES ON MATING TYPE FACTORS IN ESCHERICHIA COLI.

3. Replica plating and indirect selection of bacterial mutants.

4. Mutants of Escherichia coli requiring methionine or vitamin B12.

Review 5. Linkage map of Escherichia coli strain K-12.

6. Dual control of arabinose genes on transducing phage lambda-dara.

7. The promoter-operator region of the lac operon of Escherichia coli.

8. The dual genetic control of ornithine transcarbamylase synthesis in Escherichia coli K12.

9. Characterization of polypeptides made in vitro from bacteriophage lambda DNA.

10. Interaction of prophages at the att80 site with the chromosome of Escherichia coli.

1. Mapping of restriction sites in the attachment site region of bacteriophage lambda.

2. Transcription of the argF and argI genes of the arginine biosynthetic regulon of Escherichia coli K12, performed in vitro.

3. Metabolism of arginine-specific messenger ribonucleic acid in Escherichia coli K-12.

4. Cleavage of lambda DNA by a site-specific endonuclease from Serratia marcescens.

5. New map of bacteriophage lambda DNA.

6. Synthesis of the Escherichia coli K12 isoenzymes of ornithine transcarbamylase, performed in vitro.

7. In vitro transcription of the Escherichia coli K-12 argA, argE, and argCBH operons.

8. Molecular characterization of ilvC specialized transducing phages of Escherichia coli K-12.

9. Regulation of acetohydroxy acid synthase activities in adenyl cyclase-deficient strains of Escherichia coli K-12.