| Literature DB >> 326762 |
Abstract
Ribonucleic acid-deoxyribonucleic acid (RNA-DNA) hybridization was employed for the determination of the level of messenger RNA (mRNA) transcribed from seven of the nine genes of the arginine regulon of Escherichia coli K-12. The quantity of RNA complexing with each of the separated DNA strands of the argA, argF, argE, and argCBH operons carried on specialized transducing phages was measured. The derepressed:repressed ratio of mRNA formed in vivo was found to vary between about 3 and 4 when measured by hybridization to DNA isolated from specialized transducing phages carrying the argA, argE, argCBH, argF, and argI operons.Entities:
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Year: 1977 PMID: 326762 PMCID: PMC235412 DOI: 10.1128/jb.131.1.214-223.1977
Source DB: PubMed Journal: J Bacteriol ISSN: 0021-9193 Impact factor: 3.490