Literature DB >> 7704054

A simple method of DNA extraction from whole tissues and blood using glass powder for detection of transgenic animals by PCR.

J Attal1, M Cajero-Juarez, L M Houdebine.   

Abstract

A very simple and reliable method to extract DNA directly from mouse tail, rabbit ear and blood is described. Tissue was homogenized in a solution of NaI and the DNA was extracted using glass powder. The extracted DNA was obtained in sufficient quantity and purity to allow direct detection of transgene by PCR.

Entities:  

Mesh:

Substances:

Year:  1995        PMID: 7704054     DOI: 10.1007/bf01969417

Source DB:  PubMed          Journal:  Transgenic Res        ISSN: 0962-8819            Impact factor:   2.788


  7 in total

1.  Direct PCR of washed blood cells.

Authors:  B Y Nordvåg; G Husby; M Raafat el-Gewely
Journal:  Biotechniques       Date:  1992-04       Impact factor: 1.993

2.  Structure of the gene encoding rabbit alpha s1-casein.

Authors:  G Jolivet; E Devinoy; M L Fontaine; L M Houdebine
Journal:  Gene       Date:  1992-04-15       Impact factor: 3.688

3.  Simple procedure of DNA isolation from human serum.

Authors:  M Ishizawa; Y Kobayashi; T Miyamura; S Matsuura
Journal:  Nucleic Acids Res       Date:  1991-10-25       Impact factor: 16.971

4.  Simplified mammalian DNA isolation procedure.

Authors:  P W Laird; A Zijderveld; K Linders; M A Rudnicki; R Jaenisch; A Berns
Journal:  Nucleic Acids Res       Date:  1991-08-11       Impact factor: 16.971

5.  A fast method for high-quality genomic DNA extraction from whole human blood.

Authors:  S Gustincich; G Manfioletti; G Del Sal; C Schneider; P Carninci
Journal:  Biotechniques       Date:  1991-09       Impact factor: 1.993

6.  Direct PCR from solid tissues without DNA extraction.

Authors:  M Panaccio; M Georgesz; C Hollywell; A Lew
Journal:  Nucleic Acids Res       Date:  1993-09-25       Impact factor: 16.971

7.  A chemical method for introducing haptens onto DNA probes.

Authors:  G H Keller; C U Cumming; D P Huang; M M Manak; R Ting
Journal:  Anal Biochem       Date:  1988-05-01       Impact factor: 3.365

  7 in total
  9 in total

1.  Inside the mouse hospital: science, animal welfare and strife in the drive for AAALAC accreditation.

Authors:  V Hampshire; J Davis
Journal:  Transgenic Res       Date:  1999-08       Impact factor: 2.788

2.  Adverse effects of vapocoolant and topical anesthesia for tail biopsy of preweanling mice.

Authors:  Gillian C Braden; Angela K Brice; F Claire Hankenson
Journal:  J Am Assoc Lab Anim Sci       Date:  2015-05       Impact factor: 1.232

3.  Sensitive and universal method for microbial DNA extraction from blood products.

Authors:  N Golbang; J P Burnie; P E Klapper; A Bostock; P Williamson
Journal:  J Clin Pathol       Date:  1996-10       Impact factor: 3.411

4.  Evaluation of DNA extraction methods of rumen microbial populations.

Authors:  Gabriela Villegas-Rivera; Yevani Vargas-Cabrera; Napoleón González-Silva; Florentino Aguilera-García; Ernestina Gutiérrez-Vázquez; Alejandro Bravo-Patiño; Marcos Cajero-Juárez; Víctor Manuel Baizabal-Aguirre; Juan José Valdez-Alarcón
Journal:  World J Microbiol Biotechnol       Date:  2012-10-05       Impact factor: 3.312

5.  Identification of transgenic mice by direct PCR analysis of lysates of epithelial cells obtained from the inner surface of the rectum.

Authors:  H Lahm; A Hoeflich; N Rieger; R Wanke; E Wolf
Journal:  Transgenic Res       Date:  1998-03       Impact factor: 2.788

6.  Recombinant human extracellular superoxide dismutase produced in milk of transgenic rabbits.

Authors:  M Strömqvist; M Houdebine; J O Andersson; A Edlund; T Johansson; C Viglietta; C Puissant; L Hansson
Journal:  Transgenic Res       Date:  1997-07       Impact factor: 2.788

7.  Association of the 5'HS4 sequence of the chicken beta-globin locus control region with human EF1 alpha gene promoter induces ubiquitous and high expression of human CD55 and CD59 cDNAs in transgenic rabbits.

Authors:  F Taboit-Dameron; B Malassagne; C Viglietta; C Puissant; M Leroux-Coyau; C Chéreau; J Attal; B Weill; L M Houdebine
Journal:  Transgenic Res       Date:  1999-06       Impact factor: 2.788

8.  Unusual metabolic characteristics in skeletal muscles of transgenic rabbits for human lipoprotein lipase.

Authors:  Florence Gondret; Sanjay B Jadhao; Marie Damon; Patrick Herpin; Céline Viglietta; Louis-Marie Houdebine; Jean-François Hocquette
Journal:  Lipids Health Dis       Date:  2004-12-09       Impact factor: 3.876

9.  A Rapid and Economical Method for Efficient DNA Extraction from Diverse Soils Suitable for Metagenomic Applications.

Authors:  Selvaraju Gayathri Devi; Anwar Aliya Fathima; Sudhakar Radha; Rex Arunraj; Wayne R Curtis; Mohandass Ramya
Journal:  PLoS One       Date:  2015-07-13       Impact factor: 3.240

  9 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.