Literature DB >> 7688296

Purification of NSP1 reveals complex formation with 'GLFG' nucleoporins and a novel nuclear pore protein NIC96.

P Grandi1, V Doye, E C Hurt.   

Abstract

The essential C-terminal domain of NSP1 mediates assembly into the nuclear pore complex (NPC). To identify components which interact physically with this yeast nucleoporin, the tagged C-terminal domain of NSP1 (ProtA-NSP1) was isolated by affinity chromatography under non-denaturing conditions. The purified complex contains ProtA-NSP1, two previously identified 'GLFG' nucleoporins, NUP49 (NSP49) and p54 and a novel protein designated NIC96 (for Nucleoporin-Interacting Component of 96 kDa). Conversely, affinity purification of tagged NSP49 enriches for NSP1, the p54 and the NIC96 component. The NIC96 gene was cloned; it encodes a novel 839 amino acid protein essential for cell growth. By immunofluorescence, protein A-tagged NIC96 exhibits a punctate nuclear membrane staining indicative of nuclear pore location. Therefore, affinity purification of tagged nucleoporins has allowed the definition of a subcomplex of the NPC and analysis of physical interactions between nuclear pore proteins.

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Year:  1993        PMID： 7688296      PMCID： PMC413571          DOI： 10.1002/j.1460-2075.1993.tb05975.x

Source DB:  PubMed          Journal:  EMBO J        ISSN： 0261-4189            Impact factor:   11.598


  46 in total

1.  NSP1: a yeast nuclear envelope protein localized at the nuclear pores exerts its essential function by its carboxy-terminal domain.

Authors:  U Nehrbass; H Kern; A Mutvei; H Horstmann; B Marshallsay; E C Hurt
Journal:  Cell       Date:  1990-06-15       Impact factor: 41.582

2.  Architecture and design of the nuclear pore complex.

Authors:  J E Hinshaw; B O Carragher; R A Milligan
Journal:  Cell       Date:  1992-06-26       Impact factor: 41.582

3.  Toward a more complete 3-D structure of the nuclear pore complex.

Authors:  M Jarnik; U Aebi
Journal:  J Struct Biol       Date:  1991-12       Impact factor: 2.867

4.  Protein A-calmodulin fusions: a novel approach for investigating calmodulin function in yeast.

Authors:  D A Stirling; A Petrie; D J Pulford; D T Paterson; M J Stark
Journal:  Mol Microbiol       Date:  1992-03       Impact factor: 3.501

5.  The NUP1 gene encodes an essential component of the yeast nuclear pore complex.

Authors:  L I Davis; G R Fink
Journal:  Cell       Date:  1990-06-15       Impact factor: 41.582

6.  A major glycoprotein of the nuclear pore complex is a membrane-spanning polypeptide with a large lumenal domain and a small cytoplasmic tail.

Authors:  U F Greber; A Senior; L Gerace
Journal:  EMBO J       Date:  1990-05       Impact factor: 11.598

7.  Nuclear pore complex glycoprotein p62 of Xenopus laevis and mouse: cDNA cloning and identification of its glycosylated region.

Authors:  V Cordes; I Waizenegger; G Krohne
Journal:  Eur J Cell Biol       Date:  1991-06       Impact factor: 4.492

8.  A new family of yeast nuclear pore complex proteins.

Authors:  S R Wente; M P Rout; G Blobel
Journal:  J Cell Biol       Date:  1992-11       Impact factor: 10.539

9.  Primary sequence and heterologous expression of nuclear pore glycoprotein p62.

Authors:  C M Starr; M D'Onofrio; M K Park; J A Hanover
Journal:  J Cell Biol       Date:  1990-06       Impact factor: 10.539

10.  Nuclear protein import is inhibited by an antibody to a lumenal epitope of a nuclear pore complex glycoprotein.

Authors:  U F Greber; L Gerace
Journal:  J Cell Biol       Date:  1992-01       Impact factor: 10.539
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  93 in total

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Journal:  Genes Dev       Date:  1999-10-15       Impact factor: 11.361

2.  Purification of the vertebrate nuclear pore complex by biochemical criteria.

Authors:  B R Miller; D J Forbes
Journal:  Traffic       Date:  2000-12       Impact factor: 6.215

3.  Multiple mechanisms of suppression circumvent transcription defects in an RNA polymerase mutant.

Authors:  Q Tan; X Li; P P Sadhale; T Miyao; N A Woychik
Journal:  Mol Cell Biol       Date:  2000-11       Impact factor: 4.272

4.  Nob1p is required for cleavage of the 3' end of 18S rRNA.

Authors:  Alessandro Fatica; Marlene Oeffinger; Mensur Dlakić; David Tollervey
Journal:  Mol Cell Biol       Date:  2003-03       Impact factor: 4.272

5.  Yeast Nop15p is an RNA-binding protein required for pre-rRNA processing and cytokinesis.

Authors:  Marlene Oeffinger; David Tollervey
Journal:  EMBO J       Date:  2003-12-15       Impact factor: 11.598

6.  Ded1p, a conserved DExD/H-box translation factor, can promote yeast L-A virus negative-strand RNA synthesis in vitro.

Authors:  Jean-Leon Chong; Ray-Yuan Chuang; Luh Tung; Tien-Hsien Chang
Journal:  Nucleic Acids Res       Date:  2004-04-02       Impact factor: 16.971

7.  Architecture of the fungal nuclear pore inner ring complex.

Authors:  Tobias Stuwe; Christopher J Bley; Karsten Thierbach; Stefan Petrovic; Sandra Schilbach; Daniel J Mayo; Thibaud Perriches; Emily J Rundlet; Young E Jeon; Leslie N Collins; Ferdinand M Huber; Daniel H Lin; Marcin Paduch; Akiko Koide; Vincent Lu; Jessica Fischer; Ed Hurt; Shohei Koide; Anthony A Kossiakoff; André Hoelz
Journal:  Science       Date:  2015-08-27       Impact factor: 47.728

8.  Nup93, a vertebrate homologue of yeast Nic96p, forms a complex with a novel 205-kDa protein and is required for correct nuclear pore assembly.

Authors:  P Grandi; T Dang; N Pané; A Shevchenko; M Mann; D Forbes; E Hurt
Journal:  Mol Biol Cell       Date:  1997-10       Impact factor: 4.138

9.  Nucleoporins NPP-1, NPP-3, NPP-4, NPP-11 and NPP-13 are required for proper spindle orientation in C. elegans.

Authors:  Aaron Schetter; Peter Askjaer; Fabio Piano; Iain Mattaj; Kenneth Kemphues
Journal:  Dev Biol       Date:  2005-12-02       Impact factor: 3.582

10.  A novel complex of membrane proteins required for formation of a spherical nucleus.

Authors:  S Siniossoglou; H Santos-Rosa; J Rappsilber; M Mann; E Hurt
Journal:  EMBO J       Date:  1998-11-16       Impact factor: 11.598
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