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Literature DB >> 7684758

A new multilabeling technique for simultaneous demonstration of different islet cells in permanent slides.

Abstract

We describe a simple immunohistochemical method for simultaneous demonstration of insulin, glucagon, somatostatin, and pancreatic polypeptide cells of the Langerhans islet in routinely fixed paraffin-embedded human and Syrian hamster pancreases. With the use of alcohol insoluble chromogens, the slides can be mounted in Permount and be kept permanently without losing the color quality and intensity. With this technique, the spatial topographical distribution and morphology of the individual pancreatic endocrine cells can be investigated in the healthy and diseased pancreas.

Entities: Chemical Disease Gene Species

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Year: 1993 PMID： 7684758 DOI： 10.1007/BF02786082

Source DB: PubMed Journal: Int J Pancreatol ISSN： 0169-4197

10 in total

1. Applications of immunocolloids in light microscopy. IV. Use of photochemical silver staining in a simple and efficient double-staining technique.

Authors: C Manigley; J Roth
Journal: J Histochem Cytochem Date: 1985-12 Impact factor: 2.479

2. Localization of human pituitary hormones by multiple immunoenzyme staining procedures using monoclonal and polyclonal antibodies.

Authors: S Van Noorden; M C Stuart; A Cheung; E F Adams; J M Polak
Journal: J Histochem Cytochem Date: 1986-03 Impact factor: 2.479

3. Application of immunogold-silver staining and immunoenzymatic methods in multiple labelling of human pancreatic Langerhans islet cells.

Authors: T Krenács; Z Lászik; E Dobó
Journal: Acta Histochem Date: 1989 Impact factor: 2.479

4. Simultaneous localization of multiple tissue antigens using the peroxidase-labeled antibody method: a study on pituitary glands of the rat.

Authors: P K Nakane
Journal: J Histochem Cytochem Date: 1968-09 Impact factor: 2.479

5. Immunogold-silver staining: new method of immunostaining with enhanced sensitivity.

Authors: C S Holgate; P Jackson; P N Cowen; C C Bird
Journal: J Histochem Cytochem Date: 1983-07 Impact factor: 2.479

6. Alkaline phosphatase and peroxidase for double immunoenzymatic labelling of cellular constituents.

Authors: D Y Mason; R Sammons
Journal: J Clin Pathol Date: 1978-05 Impact factor: 3.411

7. [Immunohistochemical detection of chromogranin A and morphometric findings in pancreatic nesidioblastosis in children].

Authors: J Kunz; H Haberland; A Schmidt; P Amendt
Journal: Acta Histochem Date: 1990 Impact factor: 2.479

8. Color modification of diaminobenzidine (DAB) precipitation by metallic ions and its application for double immunohistochemistry.

Authors: S M Hsu; E Soban
Journal: J Histochem Cytochem Date: 1982-10 Impact factor: 2.479

9. Chromogranin A (CGA) in the gastro-entero-pancreatic (GEP) endocrine system. I. CGA in the mammalian endocrine pancreas.

Authors: D Grube; D Aunis; F Bader; Y Cetin; A Jörns; S Yoshie
Journal: Histochemistry Date: 1986

10. Chromogranin A in the pancreatic islet: cellular and subcellular distribution.

Authors: M Ehrhart; D Grube; M F Bader; D Aunis; M Gratzl
Journal: J Histochem Cytochem Date: 1986-12 Impact factor: 2.479

10 in total

7 in total

A new multilabeling technique for simultaneous demonstration of different islet cells in permanent slides.

1. Applications of immunocolloids in light microscopy. IV. Use of photochemical silver staining in a simple and efficient double-staining technique.

2. Localization of human pituitary hormones by multiple immunoenzyme staining procedures using monoclonal and polyclonal antibodies.

3. Application of immunogold-silver staining and immunoenzymatic methods in multiple labelling of human pancreatic Langerhans islet cells.

4. Simultaneous localization of multiple tissue antigens using the peroxidase-labeled antibody method: a study on pituitary glands of the rat.

5. Immunogold-silver staining: new method of immunostaining with enhanced sensitivity.

6. Alkaline phosphatase and peroxidase for double immunoenzymatic labelling of cellular constituents.

7. [Immunohistochemical detection of chromogranin A and morphometric findings in pancreatic nesidioblastosis in children].

8. Color modification of diaminobenzidine (DAB) precipitation by metallic ions and its application for double immunohistochemistry.

9. Chromogranin A (CGA) in the gastro-entero-pancreatic (GEP) endocrine system. I. CGA in the mammalian endocrine pancreas.

10. Chromogranin A in the pancreatic islet: cellular and subcellular distribution.

1. Experimental evidence for the origin of ductal-type adenocarcinoma from the islets of Langerhans.

2. Stimulation of islet cell proliferation enhances pancreatic ductal carcinogenesis in the hamster model.

3. A novel tissue for islet transplantation in diabetics.

4. The acute effects of pancreaticopyelostomy on the pancreas and kidney. A preliminary study.

5. The patterns of coexpression of tumor-associated antigens CA 19-9, TAG-72, and DU-PAN-2 in human pancreatic cancer.

6. The role of pancreatic islets in experimental pancreatic carcinogenicity.

7. MUC4 mucin expression in human pancreatic tumours is affected by organ environment: the possible role of TGFbeta2.