Literature DB >> 7684035

Determination in situ of neutral and acidic fucose-containing oligosaccharides in the bovine submandibular gland.

G Menghi1, D Accili, P Scocco, G Materazzi.   

Abstract

Lectins conjugated to horseradish peroxidase in combination with selected exoglycosidase digestion procedures were used to localize fucoglycoconjugates in the bovine submandibular gland. In particular, sequential treatments were employed to determine the distribution of neutral and acidic fucose-containing oligosaccharides that were previously shown to be present by biochemical techniques. Information was obtained on the distribution of the acidic oligosaccharide A-1a, alpha-Fuc(1-->2)-beta-Gal-(1-->4)-beta-GlcNAc-(1-->3)-[alpha-NeuAc-(2--> 6)]-GalNAc-ol, which was sequenced in situ and localized in acinar cells.

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Year:  1993        PMID： 7684035     DOI： 10.1007/bf00269139

Source DB:  PubMed          Journal:  Histochemistry        ISSN： 0301-5564


  22 in total

1.  Identification of muramyl derivatives in Mollusca Gastropoda tissue.

Authors:  G Menghi; E Ottaviani; D Accili; A M Bolognani Fantin
Journal:  Histochemistry       Date:  1991

2.  Comparison of the unmasking of lectin receptors by neuraminidase and by enzyme-free buffer alone.

Authors:  J Plendl; B Schönleber; W Schmahl; U Schumacher
Journal:  J Histochem Cytochem       Date:  1989-11       Impact factor: 2.479

3.  Enzymatic degradation and quantitative lectin labeling for characterizing glycoconjugates which act as lectin acceptors in cat submandibular gland.

Authors:  G Menghi; D Accili; A M Bondi; M G Gabrielli
Journal:  Histochemistry       Date:  1989

4.  Reactivity of peroxidase-labeled lectins in rabbit submandibular and sublingual glands.

Authors:  G Menghi
Journal:  Acta Histochem       Date:  1984       Impact factor: 2.479

5.  The early stages of absorption of injected horseradish peroxidase in the proximal tubules of mouse kidney: ultrastructural cytochemistry by a new technique.

Authors:  R C Graham; M J Karnovsky
Journal:  J Histochem Cytochem       Date:  1966-04       Impact factor: 2.479

6.  Lectin histochemistry of secretory and cell-surface glycoconjugates in the ovine submandibular gland.

Authors:  B A Schulte; S S Spicer; R L Miller
Journal:  Cell Tissue Res       Date:  1985       Impact factor: 5.249

7.  Carbohydrate structures of bovine submaxillary mucin.

Authors:  T Tsuji; T Osawa
Journal:  Carbohydr Res       Date:  1986-08-15       Impact factor: 2.104

8.  Determination of the structure of a novel acidic oligosaccharide with blood-group activity isolated from bovine submaxillary-gland mucin.

Authors:  S M D'Arcy; C M Donoghue; C A Koeleman; D H Van den Eijnden; A V Savage
Journal:  Biochem J       Date:  1989-06-01       Impact factor: 3.857

9.  Effects of alpha-galactosidase digestion on lectin staining in human pancreas.

Authors:  N Ito; K Nishi; M Nakajima; Y Okamura; T Hirota
Journal:  Histochemistry       Date:  1988

10.  Characterisation in situ of the complex carbohydrates in rabbit oviduct using digestion with glycosidases followed by lectin binding.

Authors:  G Menghi; A M Bondi; D Accili; L Fumagalli; G Materazzi
Journal:  J Anat       Date:  1985-06       Impact factor: 2.610
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