Literature DB >> 2439407

Method for blot-hybridization analysis of mRNA molecules from Bacillus subtilis.

N P Ambulos, E J Duvall, P S Lovett.   

Abstract

By modifying hybridization techniques which are currently available to analyze RNA molecules we have developed a sensitive and reproducible method for 'Northern' analysis of RNA from Bacillus subtilis. The use of a thin (1 mm) vertical 2% agarose-6% formaldehyde gel seems to allow more efficient transfer and higher resolution of RNA upon hybridization analysis than does the use of thicker horizontal slab gels. Our improved hybridization method results in greatly reduced background upon autoradiography regardless of whether or not 32P-labelled nick-translated probes or probes synthesized on M13 vectors were purified from the unincorporated radionucleotides.

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Year:  1987        PMID： 2439407     DOI： 10.1016/0378-1119(87)90317-9

Source DB:  PubMed          Journal:  Gene        ISSN： 0378-1119            Impact factor:   3.688


  13 in total

1.  Multinucleated giant cell formation and apoptosis in infected host cells is mediated by Burkholderia pseudomallei type III secretion protein BipB.

Authors:  Supaporn Suparak; Wannapa Kespichayawattana; Ashraful Haque; Anna Easton; Suwat Damnin; Ganjana Lertmemongkolchai; Gregory J Bancroft; Sunee Korbsrisate
Journal:  J Bacteriol       Date:  2005-09       Impact factor: 3.490

2.  Heterologous growth phase- and temperature-dependent expression and H2O2 toxicity protection of a superoxide-inducible monofunctional catalase gene from Xanthomonas oryzae pv. oryzae.

Authors:  S Mongkolsuk; S Loprasert; P Vattanaviboon; C Chanvanichayachai; S Chamnongpol; N Supsamran
Journal:  J Bacteriol       Date:  1996-06       Impact factor: 3.490

3.  Direct extraction and purification of rRNA for ecological studies.

Authors:  M A Moran; V L Torsvik; T Torsvik; R E Hodson
Journal:  Appl Environ Microbiol       Date:  1993-03       Impact factor: 4.792

4.  bmr3, a third multidrug transporter gene of Bacillus subtilis.

Authors:  R Ohki; M Murata
Journal:  J Bacteriol       Date:  1997-02       Impact factor: 3.490

5.  Darkness and antibiotics increase the steady-state transcripts of the elongation factor gene (tuf) in Chlamydomonas reinhardtii.

Authors:  G W Silk; M Wu
Journal:  Curr Genet       Date:  1988-08       Impact factor: 3.886

6.  A variation of the translation attenuation model can explain the inducible regulation of the pBC16 tetracycline resistance gene in Bacillus subtilis.

Authors:  Patricia B Lodato; Elizabeth J Rogers; Paul S Lovett
Journal:  J Bacteriol       Date:  2006-07       Impact factor: 3.490

7.  Modification of length, hydrophobic properties and electric charge of Bacillus subtilis alpha-amylase signal peptide and their different effects on the production of secretory proteins in B. subtilis and Escherichia coli cells.

Authors:  K Nakamura; Y Fujita; Y Itoh; K Yamane
Journal:  Mol Gen Genet       Date:  1989-03

8.  Cloning, sequencing, and molecular analysis of the dnaK locus from Bacillus subtilis.

Authors:  M Wetzstein; U Völker; J Dedio; S Löbau; U Zuber; M Schiesswohl; C Herget; M Hecker; W Schumann
Journal:  J Bacteriol       Date:  1992-05       Impact factor: 3.490

9.  Carbon Cycling: Molecular Regulation of Photosynthetic Carbon Fixation

Authors: 
Journal:  Microb Ecol       Date:  1996-11       Impact factor: 4.552

10.  RsbV-independent induction of the SigB-dependent general stress regulon of Bacillus subtilis during growth at high temperature.

Authors:  Gudrun Holtmann; Matthias Brigulla; Leif Steil; Alexandra Schütz; Karsta Barnekow; Uwe Völker; Erhard Bremer
Journal:  J Bacteriol       Date:  2004-09       Impact factor: 3.490
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