Literature DB >> 7683153

Epitope mapping of the major inner capsid protein of group A rotavirus using peptide synthesis.

E Kohli1, L Maurice, C Bourgeois, J B Bour, P Pothier.   

Abstract

Three hundred and ninety-one consecutive heptapeptides derived from the VP6 protein of bovine rotavirus (397 AA) were synthesized using the "pepscan" method and were assayed on the synthesis pins with monoclonal antibodies to VP6. Heptapeptides reactive with MAbs were located in four main regions: regions AA 32-64, AA 155-167, AA 208-274, and a fourth region at the C-terminal, from AA 380 to AA 397. Among these regions, two sequences were also reactive with the MAbs when longer peptides were assayed. The sequence located between AA 58 and AA 62 (NWNFD), recognized by MAbs RV-1026, RV-50, and RV-443, was previously reported. A new site was defined in the region essential for trimerization, between AA 159 and AA 165 (PYSASFT), which was recognized by MAbs RV-133 and RV-138.

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Year:  1993        PMID： 7683153     DOI： 10.1006/viro.1993.1240

Source DB:  PubMed          Journal:  Virology        ISSN： 0042-6822            Impact factor:   3.616


  10 in total

1.  Interactions of rotavirus VP4 spike protein with the endosomal protein Rab5 and the prenylated Rab acceptor PRA1.

Authors:  Vincent Enouf; Serge Chwetzoff; Germain Trugnan; Jean Cohen
Journal:  J Virol       Date:  2003-06       Impact factor: 5.103

2.  Rotavirus nonstructural protein NSP5 interacts with major core protein VP2.

Authors:  Mabel Berois; Catherine Sapin; Inge Erk; Didier Poncet; Jean Cohen
Journal:  J Virol       Date:  2003-02       Impact factor: 5.103

3.  Rotavirus virus-like particles as surrogates in environmental persistence and inactivation studies.

Authors:  Santiago Caballero; F Xavier Abad; Fabienne Loisy; Françoise S Le Guyader; Jean Cohen; Rosa M Pintó; Albert Bosch
Journal:  Appl Environ Microbiol       Date:  2004-07       Impact factor: 4.792

4.  Whole genome sequencing of lamb rotavirus and comparative analysis with other mammalian rotaviruses.

Authors:  Yanjun Chen; Weiwen Zhu; Shuo Sui; Yuxin Yin; Songnian Hu; Xiaowei Zhang
Journal:  Virus Genes       Date:  2009-02-13       Impact factor: 2.332

5.  Inhibition of in vitro reconstitution of rotavirus transcriptionally active particles by anti-VP6 monoclonal antibodies.

Authors:  E Kohli; P Pothier; G Tosser; J Cohen; A M Sandino; E Spencer
Journal:  Arch Virol       Date:  1994       Impact factor: 2.574

6.  A new rotavirus VP6-based foreign epitope presenting vector and immunoreactivity of VP4 epitope chimeric proteins.

Authors:  Yumei Teng; Bingxin Zhao; Xiaoxia Pan; Yuling Wen; Yuanding Chen
Journal:  Viral Immunol       Date:  2014-04-04       Impact factor: 2.257

7.  Sequence analysis of cDNA for the VP6 protein of group A avian rotavirus: a comparison with group A mammalian rotaviruses.

Authors:  H Ito; N Minamoto; I Sasaki; H Goto; M Sugiyama; T Kinjo; S Sugita
Journal:  Arch Virol       Date:  1995       Impact factor: 2.574

8.  Mapping of antigenic sites on the major inner capsid protein of avian rotavirus using an Escherichia coli expression system.

Authors:  H Ito; N Minamoto; H Goto; T R Luo; M Sugiyama; T Kinjo
Journal:  Arch Virol       Date:  1996       Impact factor: 2.574

9.  Oral Administration of a Seed-based Bivalent Rotavirus Vaccine Containing VP6 and NSP4 Induces Specific Immune Responses in Mice.

Authors:  Hao Feng; Xin Li; Weibin Song; Mei Duan; Hong Chen; Tao Wang; Jiangli Dong
Journal:  Front Plant Sci       Date:  2017-05-31       Impact factor: 5.753

10.  Intracellular neutralisation of rotavirus by VP6-specific IgG.

Authors:  Sarah L Caddy; Marina Vaysburd; Mark Wing; Stian Foss; Jan Terje Andersen; Kevin O'Connell; Keith Mayes; Katie Higginson; Miren Iturriza-Gómara; Ulrich Desselberger; Leo C James
Journal:  PLoS Pathog       Date:  2020-08-04       Impact factor: 6.823
  10 in total

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