Literature DB >> 7683003

Membrane translocation and channel-forming activities of diphtheria toxin are blocked by replacing isoleucine 364 with lysine.

V Cabiaux1, J Mindell, R J Collier.   

Abstract

A mutant of diphtheria toxin in which Ile-364 was replaced by Lys was at least 500-fold less toxic to Vero cells than the parental toxin. Its ability to undergo low-pH-triggered translocation across the plasma membrane was greatly diminished, as was its ability to form ion-conductive channels. In addition, the mutant toxin was inactive in the pH-dependent killing of Escherichia coli.

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Year:  1993        PMID： 7683003      PMCID： PMC280822          DOI： 10.1128/iai.61.5.2200-2202.1993

Source DB:  PubMed          Journal:  Infect Immun        ISSN： 0019-9567            Impact factor:   3.441


  13 in total

1.  Cloned diphtheria toxin within the periplasm of Escherichia coli causes lethal membrane damage at low pH.

Authors:  D O'Keefe; R J Collier
Journal:  Proc Natl Acad Sci U S A       Date:  1989-01       Impact factor: 11.205

Review 2.  Diphtheria toxin: membrane interaction and membrane translocation.

Authors:  E London
Journal:  Biochim Biophys Acta       Date:  1992-03-26

3.  The crystal structure of diphtheria toxin.

Authors:  S Choe; M J Bennett; G Fujii; P M Curmi; K A Kantardjieff; R J Collier; D Eisenberg
Journal:  Nature       Date:  1992-05-21       Impact factor: 49.962

Review 4.  Protein toxins with intracellular targets.

Authors:  S Olsnes; H Stenmark; J O Moskaug; S McGill; I H Madshus; K Sandvig
Journal:  Microb Pathog       Date:  1990-03       Impact factor: 3.738

5.  The complete amino acid sequence of diphtheria toxin fragment B. Correlation with its lipid-binding properties.

Authors:  P Falmagne; C Capiau; P Lambotte; J Zanen; V Cabiaux; J M Ruysschaert
Journal:  Biochim Biophys Acta       Date:  1985-01-21

6.  Diphtheria toxin: quantification and assay.

Authors:  S F Carroll; R J Collier
Journal:  Methods Enzymol       Date:  1988       Impact factor: 1.600

7.  Diphtheria toxin forms transmembrane channels in planar lipid bilayers.

Authors:  J J Donovan; M I Simon; R K Draper; M Montal
Journal:  Proc Natl Acad Sci U S A       Date:  1981-01       Impact factor: 11.205

8.  Rapid entry of nicked diphtheria toxin into cells at low pH. Characterization of the entry process and effects of low pH on the toxin molecule.

Authors:  K Sandvig; S Olsnes
Journal:  J Biol Chem       Date:  1981-09-10       Impact factor: 5.157

9.  Improved oligonucleotide site-directed mutagenesis using M13 vectors.

Authors:  P Carter; H Bedouelle; G Winter
Journal:  Nucleic Acids Res       Date:  1985-06-25       Impact factor: 16.971

10.  Identification of a translocated gating charge in a voltage-dependent channel. Colicin E1 channels in planar phospholipid bilayer membranes.

Authors:  C K Abrams; K S Jakes; A Finkelstein; S L Slatin
Journal:  J Gen Physiol       Date:  1991-07       Impact factor: 4.086
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  7 in total

Review 1.  Obstructing toxin pathways by targeted pore blockage.

Authors:  Ekaterina M Nestorovich; Sergey M Bezrukov
Journal:  Chem Rev       Date:  2012-10-11       Impact factor: 60.622

2.  Proton-coupled protein transport through the anthrax toxin channel.

Authors:  Alan Finkelstein
Journal:  Philos Trans R Soc Lond B Biol Sci       Date:  2009-01-27       Impact factor: 6.237

3.  Roles of Glu 349 and Asp 352 in membrane insertion and translocation by diphtheria toxin.

Authors:  P Kaul; J Silverman; W H Shen; S R Blanke; P D Huynh; A Finkelstein; R J Collier
Journal:  Protein Sci       Date:  1996-04       Impact factor: 6.725

Review 4.  Recombinant immunotoxins containing truncated bacterial toxins for the treatment of hematologic malignancies.

Authors:  Robert J Kreitman
Journal:  BioDrugs       Date:  2009       Impact factor: 5.807

5.  Structure-function relationships in diphtheria toxin channels: I. Determining a minimal channel-forming domain.

Authors:  J A Silverman; J A Mindell; H Zhan; A Finkelstein; R J Collier
Journal:  J Membr Biol       Date:  1994-01       Impact factor: 1.843

Review 6.  Mechanism of diphtheria toxin catalytic domain delivery to the eukaryotic cell cytosol and the cellular factors that directly participate in the process.

Authors:  John R Murphy
Journal:  Toxins (Basel)       Date:  2011-03-21       Impact factor: 4.546

7.  The diphtheria toxin channel-forming T domain translocates its own NH2-terminal region across planar bilayers.

Authors:  L Senzel; P D Huynh; K S Jakes; R J Collier; A Finkelstein
Journal:  J Gen Physiol       Date:  1998-09       Impact factor: 4.086
  7 in total

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