Literature DB >> 7682035

Affinity purification of antibodies specific for Asn-linked glycans containing alpha 1-->3 fucose or beta 1-->2 xylose.

L Faye1, V Gomord, A C Fitchette-Lainé, M J Chrispeels.   

Abstract

Antisera raised against the plant glycoproteins beta-fructosidase and horseradish peroxidase can be fractionated on an affinity column of honeybee venom phospholipase A2 to produce serum fractions that are specific for either the alpha 1-->3 fucose or beta 1-->2 xylose epitopes commonly found on the Asn-linked glycans of plant glycoproteins. This affinity purification strategy relies on the absence of beta 1-->2 xylose from the glycan of the venom protein. Such antibody preparations can be used for the detection of these sugar epitopes on glycoproteins.

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Year:  1993        PMID： 7682035     DOI： 10.1006/abio.1993.1088

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  36 in total

1.  Protein recycling from the Golgi apparatus to the endoplasmic reticulum in plants and its minor contribution to calreticulin retention.

Authors:  S Pagny; M Cabanes-Macheteau; J W Gillikin; N Leborgne-Castel; P Lerouge; R S Boston; L Faye; V Gomord
Journal:  Plant Cell       Date:  2000-05       Impact factor: 11.277

Review 2.  Protein N-glycosylation in the baculovirus-insect cell system.

Authors:  Xianzong Shi; Donald L Jarvis
Journal:  Curr Drug Targets       Date:  2007-10       Impact factor: 3.465

Review 3.  Crossreactive carbohydrate determinants.

Authors:  R C Aalberse; R van Ree
Journal:  Clin Rev Allergy Immunol       Date:  1997       Impact factor: 8.667

4.  Expression of functional hexahistidine-tagged ricin B in tobacco.

Authors:  Deborah G Reed; Luis H Nopo-Olazabal; Vanessa Funk; Bonnie J Woffenden; Michael J Reidy; Maureen C Dolan; Carole L Cramer; Fabricio Medina-Bolivar
Journal:  Plant Cell Rep       Date:  2004-12-15       Impact factor: 4.570

5.  Arabidopsis glucosidase I mutants reveal a critical role of N-glycan trimming in seed development.

Authors:  M Boisson; V Gomord; C Audran; N Berger; B Dubreucq; F Granier; P Lerouge; L Faye; M Caboche; L Lepiniec
Journal:  EMBO J       Date:  2001-03-01       Impact factor: 11.598

6.  Delivery of prolamins to the protein storage vacuole in maize aleurone cells.

Authors:  Francisca C Reyes; Taijoon Chung; David Holding; Rudolf Jung; Richard Vierstra; Marisa S Otegui
Journal:  Plant Cell       Date:  2011-02-22       Impact factor: 11.277

7.  Three glycosylated polypeptides secreted by several embryogenic cell cultures of pine show highly specific serological affinity to antibodies directed against the wheat germin apoprotein monomer.

Authors:  J M Domon; B Dumas; E Lainé; Y Meyer; A David; H David
Journal:  Plant Physiol       Date:  1995-05       Impact factor: 8.340

8.  Structural analysis of N-glycans from allergenic grass, ragweed and tree pollens: core alpha1,3-linked fucose and xylose present in all pollens examined.

Authors:  I B Wilson; F Altmann
Journal:  Glycoconj J       Date:  1998-11       Impact factor: 2.916

Review 9.  With or without sugar? (A)glycosylation of therapeutic antibodies.

Authors:  Dmitrij Hristodorov; Rainer Fischer; Lars Linden
Journal:  Mol Biotechnol       Date:  2013-07       Impact factor: 2.695

10.  Protein N-glycosylation is similar in the moss Physcomitrella patens and in higher plants.

Authors:  Remco Viëtor; Corinne Loutelier-Bourhis; Anne-Catherine Fitchette; Pierre Margerie; Martine Gonneau; Loïc Faye; Patrice Lerouge
Journal:  Planta       Date:  2003-10-18       Impact factor: 4.116
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