Literature DB >> 7680043

Immunohistochemical examination of tumor-suppressor gene p53 product and pyrimidine dimer in solar keratosis.

M Taguchi1, S Watanabe, Y Sato, T Kameya, N Munakata, K Ishihara, P K Nakane, H Hisatome, S Ikeda.   

Abstract

In order to find biomarkers to measure the effects of UV irradiation, we examined the accumulation of p53 protein and pyrimidine dimers in 18 solar keratosis specimens. Frozen or AMeX-fixed solar keratosis specimens were immunohistochemically stained by anti-p53 mouse monoclonal antibody, pAb1801 and polyclonal anti-(pyrimidine dimer) antibody. Nuclear accumulation of p53 protein was found in 5/18 (28%) solar keratosis lesions. The percentage of cases showing nuclear p53 protein varied according to the histological type; in the bowenoid type it was 4/7 (57%); in the atrophic type it was 1/7 (14%). Nuclear pyrimidine dimers were not stained in solar keratosis, although the skin of UV-irradiated nude mice was positive. Accumulation of p53 protein is a good marker for early precancerous change caused by UV exposure.

Entities:  

Mesh：

Substances：

Year:  1993        PMID： 7680043     DOI： 10.1007/bf01212722

Source DB:  PubMed          Journal:  J Cancer Res Clin Oncol        ISSN： 0171-5216            Impact factor:   4.553


  12 in total

1.  The AMeX method. A simplified technique of tissue processing and paraffin embedding with improved preservation of antigens for immunostaining.

Authors:  Y Sato; K Mukai; S Watanabe; M Goto; Y Shimosato
Journal:  Am J Pathol       Date:  1986-12       Impact factor: 4.307

2.  Detection of polymorphisms of human DNA by gel electrophoresis as single-strand conformation polymorphisms.

Authors:  M Orita; H Iwahana; H Kanazawa; K Hayashi; T Sekiya
Journal:  Proc Natl Acad Sci U S A       Date:  1989-04       Impact factor: 11.205

3.  In situ (6-4)photoproduct determination by laser cytometry and autoradiography.

Authors:  T Mori; T Matsunaga; C C Chang; J E Trosko; O Nikaido
Journal:  Mutat Res       Date:  1990-07       Impact factor: 2.433

4.  Comparative removal of pyrimidine dimers from human epidermal keratinocytes in vivo and in vitro.

Authors:  M K Reusch; K Meager; S A Leadon; P C Hanawalt
Journal:  J Invest Dermatol       Date:  1988-10       Impact factor: 8.551

5.  N-ras mutations in human cutaneous melanoma from sun-exposed body sites.

Authors:  L J van 't Veer; B M Burgering; R Versteeg; A J Boot; D J Ruiter; S Osanto; P I Schrier; J L Bos
Journal:  Mol Cell Biol       Date:  1989-07       Impact factor: 4.272

6.  Wild-type p53 is a cell cycle checkpoint determinant following irradiation.

Authors:  S J Kuerbitz; B S Plunkett; W V Walsh; M B Kastan
Journal:  Proc Natl Acad Sci U S A       Date:  1992-08-15       Impact factor: 11.205

7.  A role for sunlight in skin cancer: UV-induced p53 mutations in squamous cell carcinoma.

Authors:  D E Brash; J A Rudolph; J A Simon; A Lin; G J McKenna; H P Baden; A J Halperin; J Pontén
Journal:  Proc Natl Acad Sci U S A       Date:  1991-11-15       Impact factor: 11.205

8.  Increased expression of mutant forms of p53 oncogene in primary lung cancer.

Authors:  R Iggo; K Gatter; J Bartek; D Lane; A L Harris
Journal:  Lancet       Date:  1990-03-24       Impact factor: 79.321

9.  Clinicopathological significance of nuclear accumulation of tumor suppressor gene p53 product in primary lung cancer.

Authors:  H Hiyoshi; Y Matsuno; H Kato; Y Shimosato; S Hirohashi
Journal:  Jpn J Cancer Res       Date:  1992-01

10.  High incidence of p53 gene mutation in human ovarian cancer and its association with nuclear accumulation of p53 protein and tumor DNA aneuploidy.

Authors:  T Kihana; H Tsuda; S Teshima; S Okada; S Matsuura; S Hirohashi
Journal:  Jpn J Cancer Res       Date:  1992-09
View more
  1 in total

1.  Differentiation-dependent p53 regulation of nucleotide excision repair in keratinocytes.

Authors:  G Li; V C Ho; D L Mitchell; M J Trotter; V A Tron
Journal:  Am J Pathol       Date:  1997-04       Impact factor: 4.307
  1 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.