Literature DB >> 7679330

Epitope-tagging vectors designed for yeast.

P Reisdorf1, A C Maarse, B Daignan-Fornier.   

Abstract

In order to facilitate the process of epitope-tagging of yeast proteins, we have constructed two Saccharomyces cerevisiae-Escherichia coli shuttle vectors that allow fusion of a sequence encoding an epitope of the human c-myc protein at the 3' end of any gene. An example of the use of this technique is presented.

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Year:  1993        PMID： 7679330     DOI： 10.1007/bf00352019

Source DB:  PubMed          Journal:  Curr Genet        ISSN： 0172-8083            Impact factor:   3.886


  3 in total

1.  New yeast-Escherichia coli shuttle vectors constructed with in vitro mutagenized yeast genes lacking six-base pair restriction sites.

Authors:  R D Gietz; A Sugino
Journal:  Gene       Date:  1988-12-30       Impact factor: 3.688

2.  A C-terminal signal prevents secretion of luminal ER proteins.

Authors:  S Munro; H R Pelham
Journal:  Cell       Date:  1987-03-13       Impact factor: 41.582

3.  MPI1, an essential gene encoding a mitochondrial membrane protein, is possibly involved in protein import into yeast mitochondria.

Authors:  A C Maarse; J Blom; L A Grivell; M Meijer
Journal:  EMBO J       Date:  1992-10       Impact factor: 11.598
  3 in total
  7 in total

1.  A novel multiple affinity purification tag and its use in identification of proteins associated with a cyclin-CDK complex.

Authors:  S Honey; B L Schneider; D M Schieltz; J R Yates; B Futcher
Journal:  Nucleic Acids Res       Date:  2001-02-15       Impact factor: 16.971

2.  Biogenesis of a heterologous amino acid permease expressed in saccharomyces cerevisiae.

Authors:  A Matĕjcková; H Sychrová
Journal:  Folia Microbiol (Praha)       Date:  1997       Impact factor: 2.099

3.  Two adjacent nuclear genes, ISF1 and NAM7/UPF1, cooperatively participate in mitochondrial functions in Saccharomyces cerevisiae.

Authors:  N Altamura; G Dujardin; O Groudinsky; P P Slonimski
Journal:  Mol Gen Genet       Date:  1994-01

4.  Single point mutations in domain II of the yeast mitochondrial release factor mRF-1 affect ribosome binding.

Authors:  H J Pel; M Rep; H J Dubbink; L A Grivell
Journal:  Nucleic Acids Res       Date:  1993-11-25       Impact factor: 16.971

5.  Sugar-binding activity of pea (Pisum sativum) lectin is essential for heterologous infection of transgenic white clover hairy roots by Rhizobium leguminosarum biovar viciae.

Authors:  R van Eijsden; C L Díaz; B S de Pater; J W Kijne
Journal:  Plant Mol Biol       Date:  1995-11       Impact factor: 4.076

6.  Yeast aminopeptidase I is post-translationally sorted from the cytosol to the vacuole by a mechanism mediated by its bipartite N-terminal extension.

Authors:  B Seguí-Real; M Martinez; I V Sandoval
Journal:  EMBO J       Date:  1995-11-15       Impact factor: 11.598

7.  Peptides from the SARS-associated coronavirus as tags for protein expression and purification.

Authors:  Steffi Gruschke; Bianca M Bussmann; Sven Reiche; Christian Jassoy
Journal:  Protein Expr Purif       Date:  2008-05-09       Impact factor: 1.650
  7 in total

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