Literature DB >> 7670601

Introduction of DNA into sea urchin eggs by particle gun.

K Akasaka1, A Nishimura, K Hijikata, Y Iuchi, J Morokuma, M Takahashi, H Morikawa, H Shimada.   

Abstract

We have applied the particle gun method, which was developed to introduce DNA into plant cells, to sea urchin eggs and have obtained excellent expression of the introduced DNA in the embryos. The expression can be normalized by concomitantly-introducing a reference construct. The method provides a new approach to quantitative analysis of cis-regulatory elements in sea urchin embryos.

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Year:  1995        PMID： 7670601

Source DB:  PubMed          Journal:  Mol Mar Biol Biotechnol        ISSN： 1053-6426


  3 in total

1.  Functional interaction between TATA and upstream CACGTG elements regulates the temporally specific expression of Otx mRNAs during early embryogenesis of the sea urchin, Hemicentrotus pulcherrimus.

Authors:  Akiko Kobayashi; Koji Akasaka; Masashi Kawaichi; Tetsuro Kokubo
Journal:  Nucleic Acids Res       Date:  2002-07-15       Impact factor: 16.971

2.  Ars insulator protects transgenes from long-term silencing in sea urchin larva.

Authors:  Mamiko Yajima; Masato Kiyomoto; Koji Akasaka
Journal:  Dev Genes Evol       Date:  2007-03-20       Impact factor: 0.900

3.  Structure, expression, and transcriptional regulation of the Strongylocentrotus franciscanus spec gene family encoding intracellular calcium-binding proteins.

Authors:  Jeffrey T Villinski; Takae Kiyama; Sandeep Dayal; Ning Zhang; Shuguang Liang; William H Klein
Journal:  Dev Genes Evol       Date:  2005-05-04       Impact factor: 0.900
  3 in total

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