Literature DB >> 7665495

Characterization of a new cell-bound alpha-amylase in Bacillus subtilis 168 Marburg that is only immunologically related to the exocellular alpha-amylase.

E Haddaoui1, M F Petit-Glatron, R Chambert.   

Abstract

Immunoblot analysis of Bacillus subtilis cell extracts with polyclonal antibodies, raised against purified exocellular alpha-amylase, revealed one protein species of 82,000 Da. This protein was found even in cells in which the amyE gene, encoding exocellular alpha-amylase, was disrupted. Isolated from the membrane fraction, the 82,000-M(r) protein displayed an alpha-amylase activity in vitro.

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Year:  1995        PMID： 7665495      PMCID： PMC177296          DOI： 10.1128/jb.177.17.5148-5150.1995

Source DB:  PubMed          Journal:  J Bacteriol        ISSN： 0021-9193            Impact factor:   3.490


  9 in total

1.  Membrane-bound and soluble extracellular alpha-amylase from Bacillus subtilis.

Authors:  P Mäntsälä; H Zalkin
Journal:  J Biol Chem       Date:  1979-09-10       Impact factor: 5.157

2.  Modulation of Bacillus subtilis levansucrase gene expression by sucrose and regulation of the steady-state mRNA level by sacU and sacQ genes.

Authors:  H Shimotsu; D J Henner
Journal:  J Bacteriol       Date:  1986-10       Impact factor: 3.490

3.  A neomycin resistance gene cassette selectable in a single copy state in the Bacillus subtilis chromosome.

Authors:  M Itaya; K Kondo; T Tanaka
Journal:  Nucleic Acids Res       Date:  1989-06-12       Impact factor: 16.971

4.  Nucleotide sequence of the amylase gene from Bacillus subtilis.

Authors:  M Yang; A Galizzi; D Henner
Journal:  Nucleic Acids Res       Date:  1983-01-25       Impact factor: 16.971

5.  Secretion of Bacillus subtilis levansucrase: a possible two-step mechanism.

Authors:  M F Petit-Glatron; F Benyahia; R Chambert
Journal:  Eur J Biochem       Date:  1987-03-02

6.  Signal peptide of Bacillus subtilis alpha-amylase.

Authors:  H Sasamoto; K Nakazawa; K Tsutsumi; K Takase; K Yamane
Journal:  J Biochem       Date:  1989-09       Impact factor: 3.387

7.  Construction of a Bacillus subtilis double mutant deficient in extracellular alkaline and neutral proteases.

Authors:  F Kawamura; R H Doi
Journal:  J Bacteriol       Date:  1984-10       Impact factor: 3.490

8.  Hyperproduction of exocellular levansucrase by Bacillus subtilis: examination of the phenotype of a sacUh strain.

Authors:  R Chambert; M F Petit-Glatron
Journal:  J Gen Microbiol       Date:  1984-12

9.  Genetic and biochemical studies on cell-bound alpha-amylase in Bacillus subtilis Marburg.

Authors:  Y Nagata; K Yamaguchi; B Maruo
Journal:  J Bacteriol       Date:  1974-08       Impact factor: 3.490
  9 in total

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