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Literature DB >> 7659528

Whole genome amplification of single cells: mathematical analysis of PEP and tagged PCR.

Abstract

We construct a mathematical model for two whole genome amplification strategies, primer extension preamplification (PEP) and tagged polymerase chain reaction (tagged PCR). An explicit formula for the expected target yield of PEP is obtained. The distribution of the target yield and the coverage properties of these two strategies are studied by simulations. From our studies we find that polymerase with high processivity may increase the efficiency of PEP and tagged PCR.

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Year: 1995 PMID： 7659528 PMCID： PMC307146 DOI： 10.1093/nar/23.15.3034

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

7 in total

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Journal: Proc Natl Acad Sci U S A Date: 1992-04-01 Impact factor: 11.205

2. Degenerate oligonucleotide-primed PCR: general amplification of target DNA by a single degenerate primer.

Authors: H Telenius; N P Carter; C E Bebb; M Nordenskjöld; B A Ponder; A Tunnacliffe
Journal: Genomics Date: 1992-07 Impact factor: 5.736

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Authors: L Zhang; X Cui; K Schmitt; R Hubert; W Navidi; N Arnheim
Journal: Proc Natl Acad Sci U S A Date: 1992-07-01 Impact factor: 11.205

4. Cloning defined regions of the human genome by microdissection of banded chromosomes and enzymatic amplification.

Authors: H J Lüdecke; G Senger; U Claussen; B Horsthemke
Journal: Nature Date: 1989-03-23 Impact factor: 49.962

5. Happy mapping: linkage mapping using a physical analogue of meiosis.

Authors: P H Dear; P R Cook
Journal: Nucleic Acids Res Date: 1993-01-11 Impact factor: 16.971

6. PCR amplification of megabase DNA with tagged random primers (T-PCR).

Authors: D Grothues; C R Cantor; C L Smith
Journal: Nucleic Acids Res Date: 1993-03-11 Impact factor: 16.971

7. Preimplantation single cell analyses of dystrophin gene deletions using whole genome amplification.

Authors: K Kristjansson; S S Chong; I B Van den Veyver; S Subramanian; M C Snabes; M R Hughes
Journal: Nat Genet Date: 1994-01 Impact factor: 38.330

7 in total

8 in total

Review 1. Freezer anthropology: new uses for old blood.

Authors: D A Merriwether
Journal: Philos Trans R Soc Lond B Biol Sci Date: 1999-01-29 Impact factor: 6.237

2. Use of tagged random hexamer amplification (TRHA) to clone and sequence minute quantities of DNA--application to a 180 kb plasmid isolated from Sphingomonas F199.

Authors: K K Wong; L C Stillwell; C A Dockery; J D Saffer
Journal: Nucleic Acids Res Date: 1996-10-01 Impact factor: 16.971

3. Whole genome amplification using a degenerate oligonucleotide primer allows hundreds of genotypes to be performed on less than one nanogram of genomic DNA.

Authors: V G Cheung; S F Nelson
Journal: Proc Natl Acad Sci U S A Date: 1996-12-10 Impact factor: 11.205

7. Whole genome amplification of DNA from laser capture-microdissected tissue for high-throughput single nucleotide polymorphism and short tandem repeat genotyping.

Authors: Martha S Rook; Scott M Delach; Galina Deyneko; Andrew Worlock; Jia Liu Wolfe
Journal: Am J Pathol Date: 2004-01 Impact factor: 4.307

8. Confidence intervals for Markov chain transition probabilities based on next generation sequencing reads data.

Authors: Lin Wan; Xin Kang; Jie Ren; Fengzhu Sun
Journal: Quant Biol Date: 2020-05-25