Literature DB >> 1631067

Whole genome amplification from a single cell: implications for genetic analysis.

L Zhang1, X Cui, K Schmitt, R Hubert, W Navidi, N Arnheim.   

Abstract

We have developed an in vitro method for amplifying a large fraction of the DNA sequences present in a single haploid cell by repeated primer extensions using a mixture of 15-base random oligonucleotides. We studied 12 genetic loci and estimate that the probability of amplifying any sequence in the genome to a minimum of 30 copies is not less than 0.78 (95% confidence). Whole genome amplification beginning with a single cell, or other samples with very small amounts of DNA, has significant implications for multipoint mapping by sperm or oocyte typing and possibly for genetic disease diagnosis, forensics, and the analysis of ancient DNA samples.

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Year:  1992        PMID： 1631067      PMCID： PMC49394          DOI： 10.1073/pnas.89.13.5847

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  24 in total

1.  A multiple-tubes approach for accurate genotyping of very small DNA samples by using PCR: statistical considerations.

Authors:  W Navidi; N Arnheim; M S Waterman
Journal:  Am J Hum Genet       Date:  1992-02       Impact factor: 11.025

Review 2.  PCR analysis of DNA sequences in single cells: single sperm gene mapping and genetic disease diagnosis.

Authors:  N Arnheim; H H Li; X F Cui
Journal:  Genomics       Date:  1990-11       Impact factor: 5.736

3.  Enzymatic amplification of beta-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia.

Authors:  R K Saiki; S Scharf; F Faloona; K B Mullis; G T Horn; H A Erlich; N Arnheim
Journal:  Science       Date:  1985-12-20       Impact factor: 47.728

4.  Multilocus ordering strategies based on sperm typing.

Authors:  T M Goradia; K Lange
Journal:  Ann Hum Genet       Date:  1990-01       Impact factor: 1.670

5.  Single-sperm typing: determination of genetic distance between the G gamma-globin and parathyroid hormone loci by using the polymerase chain reaction and allele-specific oligomers.

Authors:  X F Cui; H H Li; T M Goradia; K Lange; H H Kazazian; D Galas; N Arnheim
Journal:  Proc Natl Acad Sci U S A       Date:  1989-12       Impact factor: 11.205

6.  Biopsy of human preimplantation embryos and sexing by DNA amplification.

Authors:  A H Handyside; J K Pattinson; R J Penketh; J D Delhanty; R M Winston; E G Tuddenham
Journal:  Lancet       Date:  1989-02-18       Impact factor: 79.321

7.  Amplification and analysis of DNA sequences in single human sperm and diploid cells.

Authors:  H H Li; U B Gyllensten; X F Cui; R K Saiki; H A Erlich; N Arnheim
Journal:  Nature       Date:  1988-09-29       Impact factor: 49.962

8.  Ordering three DNA polymorphisms on human chromosome 3 by sperm typing.

Authors:  T M Goradia; V P Stanton; X F Cui; H Aburatani; H H Li; K Lange; D E Housman; N Arnheim
Journal:  Genomics       Date:  1991-07       Impact factor: 5.736

9.  Using PCR in preimplantation genetic disease diagnosis.

Authors:  W Navidi; N Arnheim
Journal:  Hum Reprod       Date:  1991-07       Impact factor: 6.918

10.  Sperm typing allows accurate measurement of the recombination fraction between D3S2 and D3S3 on the short arm of human chromosome 3.

Authors:  R Hubert; V P Stanton; H Aburatani; J Warren; H Li; D E Housman; N Arnheim
Journal:  Genomics       Date:  1992-04       Impact factor: 5.736
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  248 in total

1.  Simultaneous single-cell detection of two mutations for cystic fibrosis.

Authors:  K C Drury; M C Liu; W Zheng; S Kipersztok; R S Williams
Journal:  J Assist Reprod Genet       Date:  2000-10       Impact factor: 3.412

2.  Digital PCR.

Authors:  B Vogelstein; K W Kinzler
Journal:  Proc Natl Acad Sci U S A       Date:  1999-08-03       Impact factor: 11.205

Review 3.  High-throughput SNP genotyping with the Masscode system.

Authors:  M Kokoris; K Dix; K Moynihan; J Mathis; B Erwin; P Grass; B Hines; A Duesterhoeft
Journal:  Mol Diagn       Date:  2000-12

4.  A high-resolution HAPPY map of Dictyostelium discoideum chromosome 6.

Authors:  B A Konfortov; H M Cohen; A T Bankier; P H Dear
Journal:  Genome Res       Date:  2000-11       Impact factor: 9.043

5.  Single nucleotide polymorphism array analysis of flow-sorted epithelial cells from frozen versus fixed tissues for whole genome analysis of allelic loss in breast cancer.

Authors:  Elizabeth L Schubert; Li Hsu; Laura A Cousens; Jeri Glogovac; Steve Self; Brian J Reid; Peter S Rabinovitch; Peggy L Porter
Journal:  Am J Pathol       Date:  2002-01       Impact factor: 4.307

6.  Delivery and storage of single embryos, sperm, or cells in microglass capillaries.

Authors:  Min-Kang Wang; Ji-Long Liu; Li Lian; Da-Yuan Chen
Journal:  In Vitro Cell Dev Biol Anim       Date:  2002-02       Impact factor: 2.416

7.  Comprehensive human genome amplification using multiple displacement amplification.

Authors:  Frank B Dean; Seiyu Hosono; Linhua Fang; Xiaohong Wu; A Fawad Faruqi; Patricia Bray-Ward; Zhenyu Sun; Qiuling Zong; Yuefen Du; Jing Du; Mark Driscoll; Wanmin Song; Stephen F Kingsmore; Michael Egholm; Roger S Lasken
Journal:  Proc Natl Acad Sci U S A       Date:  2002-04-16       Impact factor: 11.205

Review 8.  Molecular diagnostics in preimplantation genetic diagnosis.

Authors:  Alan R Thornhill; Karen Snow
Journal:  J Mol Diagn       Date:  2002-02       Impact factor: 5.568

9.  Loss of heterozygosity analysis using whole genome amplification, cell sorting, and fluorescence-based PCR.

Authors:  T G Paulson; P C Galipeau; B J Reid
Journal:  Genome Res       Date:  1999-05       Impact factor: 9.043

10.  SNP genotyping on a genome-wide amplified DOP-PCR template.

Authors:  Struan F A Grant; Simone Steinlicht; Ulrike Nentwich; Rainer Kern; Barbara Burwinkel; Ralf Tolle
Journal:  Nucleic Acids Res       Date:  2002-11-15       Impact factor: 16.971
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