Literature DB >> 7657725

An antisense oligodeoxyribonucleotide to m-calpain mRNA inhibits myoblast fusion.

D Balcerzak1, S Poussard, J J Brustis, N Elamrani, M Soriano, P Cottin, A Ducastaing.   

Abstract

Previous studies have led to the hypothesis of a possible role for m-calpain (EC 3.4.22.17) in myoblast fusion in culture in vitro. To support this hypothesis, an antisense strategy has been used with cultured primary rat myoblasts. Using an appropriate antisense oligodeoxyribonucleotide to m-calpain mRNA, an inhibition of myoblast fusion has been observed, the maximum being obtained when the cell culture was treated with 30 microM of oligomer. Synthesis of m-calpain was decreased by 48% while high concentrations of antisense oligonucleotide do not significantly affect myoblast proliferation. The specificity of m-calpain intervention during fusion has also been confirmed using antisense oligonucleotides to mu-calpain and p94 mRNAs, respectively.

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Year:  1995        PMID： 7657725     DOI： 10.1242/jcs.108.5.2077

Source DB:  PubMed          Journal:  J Cell Sci        ISSN： 0021-9533            Impact factor:   5.285


  17 in total

1.  Disruption of the murine calpain small subunit gene, Capn4: calpain is essential for embryonic development but not for cell growth and division.

Authors:  J S Arthur; J S Elce; C Hegadorn; K Williams; P A Greer
Journal:  Mol Cell Biol       Date:  2000-06       Impact factor: 4.272

2.  Changes in intracellular calpastatin localization are mediated by reversible phosphorylation.

Authors:  M Averna; R de Tullio; M Passalacqua; F Salamino; S Pontremoli; E Melloni
Journal:  Biochem J       Date:  2001-02-15       Impact factor: 3.857

3.  Characterization of the calcium-dependent proteolytic system in a mouse muscle cell line.

Authors:  Elise Dargelos; Stephane Dedieu; Catherine Moyen; Sylvie Poussard; Philippe Veschambre; Jean-Jacques Brustis; Patrick Cottin
Journal:  Mol Cell Biochem       Date:  2002-02       Impact factor: 3.396

4.  Association between IGF-IR, m-calpain and UCP-3 gene polymorphisms and growth traits in Nanyang cattle.

Authors:  Runfeng Zhang; Xiaofeng Li
Journal:  Mol Biol Rep       Date:  2010-09-21       Impact factor: 2.316

5.  Constant expression of mouse calpastatin isoforms during differentiation in myoblast cell line, C2C12.

Authors:  K Hitomi; M Murase; T Kawamura; M Maki
Journal:  Cytotechnology       Date:  2000-07       Impact factor: 2.058

Review 6.  Proteasome and myogenesis.

Authors:  F Gardrat; V Montel; J Raymond; J L Azanza
Journal:  Mol Biol Rep       Date:  1997-03       Impact factor: 2.316

Review 7.  Proteolytic fragmentation of inositol 1,4,5-trisphosphate receptors: a novel mechanism regulating channel activity?

Authors:  Liwei Wang; Kamil J Alzayady; David I Yule
Journal:  J Physiol       Date:  2015-12-07       Impact factor: 5.182

8.  A novel polymorphisms in intron 12 of the bovine calpastatin gene.

Authors:  Edyta Juszczuk-Kubiak; Joanna Wyszyńska-Koko; Krystyna Wicińska; Stanisław Rosochacki
Journal:  Mol Biol Rep       Date:  2007-01-09       Impact factor: 2.316

9.  Three calpain isoforms are autolyzed in rat fast-twitch muscle after eccentric contractions.

Authors:  Keita Kanzaki; Mai Kuratani; Satoshi Matsunaga; Noriyuki Yanaka; Masanobu Wada
Journal:  J Muscle Res Cell Motil       Date:  2014-02-21       Impact factor: 2.698

10.  Myristoylated alanine-rich C kinase substrate (MARCKS) is involved in myoblast fusion through its regulation by protein kinase Calpha and calpain proteolytic cleavage.

Authors:  Sandrine Dulong; Sebastien Goudenege; Karine Vuillier-Devillers; Stéphane Manenti; Sylvie Poussard; Patrick Cottin
Journal:  Biochem J       Date:  2004-09-15       Impact factor: 3.857
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