Literature DB >> 7646043

Spectrofluorometric assay for rapid detection of total and fecal coliforms from surface water.

S J Park1, E J Lee, D H Lee, S H Lee, S J Kim.   

Abstract

With a spectrofluorometer, the length of the incubation time required in the fluorogenic assay was reduced to 12 h. The threshold emissions for reading the fluorogenic reaction by the spectrofluorometer were 5 and 10 U for lauryl tryptose broth media containing 4-methylumbelliferyl-beta-D-galactoside and 4-methylumbelliferyl-beta-D-glucuronide, respectively. These two kinds of threshold units were equivalent to known concentrations of free 4-methylumbelliferone of 2.5 and 6 microM, respectively, in lauryl tryptose broth media.

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Year:  1995        PMID： 7646043      PMCID： PMC167470          DOI： 10.1128/aem.61.5.2027-2029.1995

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  11 in total

1.  Enumeration of total coliforms and Escherichia coli from source water by the defined substrate technology.

Authors:  S C Edberg; M J Allen; D B Smith; N J Kriz
Journal:  Appl Environ Microbiol       Date:  1990-02       Impact factor: 4.792

2.  Rapid fluorogenic enumeration of Escherichia coli in selected, naturally contaminated high moisture foods.

Authors:  P L Poelma; C R Wilson; W H Andrews
Journal:  J Assoc Off Anal Chem       Date:  1987 Nov-Dec

3.  Assay for beta-glucuronidase in species of the genus Escherichia and its applications for drinking-water analysis.

Authors:  E W Rice; M J Allen; D J Brenner; S C Edberg
Journal:  Appl Environ Microbiol       Date:  1991-02       Impact factor: 4.792

4.  Efficacy of beta-glucuronidase assay for identification of Escherichia coli by the defined-substrate technology.

Authors:  E W Rice; M J Allen; S C Edberg
Journal:  Appl Environ Microbiol       Date:  1990-05       Impact factor: 4.792

5.  Fluorogenic assays for immediate confirmation of Escherichia coli.

Authors:  P C Feng; P A Hartman
Journal:  Appl Environ Microbiol       Date:  1982-06       Impact factor: 4.792

6.  Evaluation of colilert-marine water for detection of total coliforms and Escherichia coli in the marine environment.

Authors:  C J Palmer; Y L Tsai; A L Lang; L R Sangermano
Journal:  Appl Environ Microbiol       Date:  1993-03       Impact factor: 4.792

7.  Comparative study of commercial 4-methylumbelliferyl-beta-D-glucuronide preparations with the Standard Methods membrane filtration fecal coliform test for the detection of Escherichia coli in water samples.

Authors:  D L Clark; B B Milner; M H Stewart; R L Wolfe; B H Olson
Journal:  Appl Environ Microbiol       Date:  1991-05       Impact factor: 4.792

8.  Fluorogenic assay for rapid detection of Escherichia coli in chilled and frozen foods: collaborative study.

Authors:  L J Moberg; M K Wagner; L A Kellen
Journal:  J Assoc Off Anal Chem       Date:  1988 May-Jun

9.  New medium for the simultaneous detection of total coliforms and Escherichia coli in water.

Authors:  K P Brenner; C C Rankin; Y R Roybal; G N Stelma; P V Scarpino; A P Dufour
Journal:  Appl Environ Microbiol       Date:  1993-11       Impact factor: 4.792

10.  National field evaluation of a defined substrate method for the simultaneous detection of total coliforms and Escherichia coli from drinking water: comparison with presence-absence techniques.

Authors:  S C Edberg; M J Allen; D B Smith
Journal:  Appl Environ Microbiol       Date:  1989-04       Impact factor: 4.792
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  1 in total

1.  Comparison of commercially available kits with standard methods for the detection of coliforms and Escherichia coli in foods.

Authors:  K Venkateswaran; A Murakoshi; M Satake
Journal:  Appl Environ Microbiol       Date:  1996-07       Impact factor: 4.792
  1 in total

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