Literature DB >> 7638440

Advantage of a rapid extraction method of HIV1 DNA suitable for polymerase chain reaction.

C Vignoli1, X de Lamballerie, C Zandotti, C Tamalet, P de Micco.   

Abstract

We describe a new protocol for extraction of DNA suitable for HIV1 gene amplification from clinical samples using "Chelex-100" chelating resin. Comparison was made with the classic proteinase K extraction method; 154 specimens were extracted with both methods and subjected to PCR (polymerase chain reaction). The Chelex-100 procedure optimized the yield of DNA recovery and minimized contamination due to sample manipulation. It decreased false negative results due to PCR inhibitors. A DNA sample suitable for use in PCR was obtained in 30 minutes. Chelex-100 treatment is a simple, rapid and low-cost method for DNA extraction in clinical laboratories.

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Year:  1995        PMID： 7638440     DOI： 10.1016/0923-2516(96)81085-5

Source DB:  PubMed          Journal:  Res Virol        ISSN： 0923-2516


  6 in total

1.  Detection of Herpes simplex virus DNA by real-time PCR.

Authors:  H H Kessler; G Mühlbauer; B Rinner; E Stelzl; A Berger; H W Dörr; B Santner; E Marth; H Rabenau
Journal:  J Clin Microbiol       Date:  2000-07       Impact factor: 5.948

2.  Functional haplotypes of Fc gamma (Fcγ) receptor (FcγRIIA and FcγRIIIB) predict risk to repeated episodes of severe malarial anemia and mortality in Kenyan children.

Authors:  Collins Ouma; Gregory C Davenport; Steven Garcia; Prakasha Kempaiah; Ateefa Chaudhary; Tom Were; Samuel B Anyona; Evans Raballah; Stephen N Konah; James B Hittner; John M Vulule; John M Ong'echa; Douglas J Perkins
Journal:  Hum Genet       Date:  2011-08-05       Impact factor: 4.132

3.  Rapid detection of Mycoplasma pneumoniae by an assay based on PCR and probe hybridization in a nonradioactive microwell plate format.

Authors:  H H Kessler; D E Dodge; K Pierer; K K Young; Y Liao; B I Santner; E Eber; M G Roeger; D Stuenzner; B Sixl-Voigt; E Marth
Journal:  J Clin Microbiol       Date:  1997-06       Impact factor: 5.948

4.  Improved method of detection and molecular typing of Borrelia burgdorferi sensu lato in clinical samples by polymerase chain reaction without DNA purification.

Authors:  N Rudenko; M Golovchenko; J Nemec; J Volkaert; N Mallátová; L Grubhoffer
Journal:  Folia Microbiol (Praha)       Date:  2005       Impact factor: 2.099

5.  Detection of CMV DNA in bone marrow transplant recipients: plasma versus leucocyte polymerase chain reaction.

Authors:  P C Woo; S K Lo; K Y Yuen; J S Peiris; H Siau; E K Chiu; R H Liang; T K Chan
Journal:  J Clin Pathol       Date:  1997-03       Impact factor: 3.411

6.  Rapid detection of HIV-1 proviral DNA for early infant diagnosis using recombinase polymerase amplification.

Authors:  David S Boyle; Dara A Lehman; Lorraine Lillis; Dylan Peterson; Mitra Singhal; Niall Armes; Mathew Parker; Olaf Piepenburg; Julie Overbaugh
Journal:  MBio       Date:  2013-04-02       Impact factor: 7.867
  6 in total

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