Literature DB >> 7637039

Efficient magnesium-dependent human immunodeficiency virus type 1 integrase activity.

A Engelman1, R Craigie.   

Abstract

The integrase protein from human immunodeficiency virus type 1 (HIV-1) has generally been reported to require Mn2+ for efficient in vitro activity. We have reexamined the divalent metal ion requirements of HIV-1 integrase and find that the protein is capable of promoting efficient 3' processing and DNA strand transfer with either Mn2+ or Mg2+. The metal ion preference depended upon the reaction conditions. HIV-1 integrase displayed significantly less nonspecific nuclease activity in reaction mixtures containing Mg2+ than it did under the previously described reaction conditions with mixtures containing Mn2+.

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Year:  1995        PMID： 7637039      PMCID： PMC189473     

Source DB:  PubMed          Journal:  J Virol        ISSN： 0022-538X            Impact factor:   5.103


  24 in total

1.  Concerted integration of viral DNA termini by purified avian myeloblastosis virus integrase.

Authors:  M L Fitzgerald; A C Vora; W G Zeh; D P Grandgenett
Journal:  J Virol       Date:  1992-11       Impact factor: 5.103

2.  Identification of conserved amino acid residues critical for human immunodeficiency virus type 1 integrase function in vitro.

Authors:  A Engelman; R Craigie
Journal:  J Virol       Date:  1992-11       Impact factor: 5.103

Review 3.  Macromolecular crowding: biochemical, biophysical, and physiological consequences.

Authors:  S B Zimmerman; A P Minton
Journal:  Annu Rev Biophys Biomol Struct       Date:  1993

4.  Integration of human immunodeficiency virus type 1 DNA in vitro.

Authors:  C M Farnet; W A Haseltine
Journal:  Proc Natl Acad Sci U S A       Date:  1990-06       Impact factor: 11.205

5.  Identification of amino acid residues critical for endonuclease and integration activities of HIV-1 IN protein in vitro.

Authors:  M Drelich; R Wilhelm; J Mous
Journal:  Virology       Date:  1992-06       Impact factor: 3.616

6.  The IN protein of Moloney murine leukemia virus processes the viral DNA ends and accomplishes their integration in vitro.

Authors:  R Craigie; T Fujiwara; F Bushman
Journal:  Cell       Date:  1990-08-24       Impact factor: 41.582

7.  Mutational analysis of the integrase protein of human immunodeficiency virus type 2.

Authors:  D C van Gent; A A Groeneger; R H Plasterk
Journal:  Proc Natl Acad Sci U S A       Date:  1992-10-15       Impact factor: 11.205

8.  Requirement of active human immunodeficiency virus type 1 integrase enzyme for productive infection of human T-lymphoid cells.

Authors:  R L LaFemina; C L Schneider; H L Robbins; P L Callahan; K LeGrow; E Roth; W A Schleif; E A Emini
Journal:  J Virol       Date:  1992-12       Impact factor: 5.103

9.  Characterization of human immunodeficiency virus type 1 integrase expressed in Escherichia coli and analysis of variants with amino-terminal mutations.

Authors:  K A Vincent; V Ellison; S A Chow; P O Brown
Journal:  J Virol       Date:  1993-01       Impact factor: 5.103

10.  Multiple effects of mutations in human immunodeficiency virus type 1 integrase on viral replication.

Authors:  A Engelman; G Englund; J M Orenstein; M A Martin; R Craigie
Journal:  J Virol       Date:  1995-05       Impact factor: 5.103
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  28 in total

1.  Rapid microtiter assays for poxvirus topoisomerase, mammalian type IB topoisomerase and HIV-1 integrase: application to inhibitor isolation.

Authors:  Y Hwang; D Rhodes; F Bushman
Journal:  Nucleic Acids Res       Date:  2000-12-15       Impact factor: 16.971

2.  Human immunodeficiency virus type 1 integrase: arrangement of protein domains in active cDNA complexes.

Authors:  K Gao; S L Butler; F Bushman
Journal:  EMBO J       Date:  2001-07-02       Impact factor: 11.598

3.  Human immunodeficiency virus type 1 nucleocapsid protein specifically stimulates Mg2+-dependent DNA integration in vitro.

Authors:  S Carteau; S C Batson; L Poljak; J F Mouscadet; H de Rocquigny; J L Darlix; B P Roques; E Käs; C Auclair
Journal:  J Virol       Date:  1997-08       Impact factor: 5.103

4.  Correct integration of model substrates by Ty1 integrase.

Authors:  S P Moore; D J Garfinkel
Journal:  J Virol       Date:  2000-12       Impact factor: 5.103

5.  Solution conformation and dynamics of the HIV-1 integrase core domain.

Authors:  Nicholas C Fitzkee; James E Masse; Yang Shen; David R Davies; Ad Bax
Journal:  J Biol Chem       Date:  2010-04-01       Impact factor: 5.157

6.  Correlation of recombinant integrase activity and functional preintegration complex formation during acute infection by replication-defective integrase mutant human immunodeficiency virus.

Authors:  Xiang Li; Yasuhiro Koh; Alan Engelman
Journal:  J Virol       Date:  2012-01-25       Impact factor: 5.103

7.  A mutation in integrase can compensate for mutations in the simian immunodeficiency virus att site.

Authors:  Z Du; P O Ilyinskii; K Lally; R C Desrosiers; A Engelman
Journal:  J Virol       Date:  1997-11       Impact factor: 5.103

8.  Sequence specificity of viral end DNA binding by HIV-1 integrase reveals critical regions for protein-DNA interaction.

Authors:  D Esposito; R Craigie
Journal:  EMBO J       Date:  1998-10-01       Impact factor: 11.598

9.  Differential inhibition of HIV-1 preintegration complexes and purified integrase protein by small molecules.

Authors:  C M Farnet; B Wang; J R Lipford; F D Bushman
Journal:  Proc Natl Acad Sci U S A       Date:  1996-09-03       Impact factor: 11.205

10.  Nonspecific alcoholysis, a novel endonuclease activity of human immunodeficiency virus type 1 and other retroviral integrases.

Authors:  M Katzman; M Sudol
Journal:  J Virol       Date:  1996-04       Impact factor: 5.103
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