Literature DB >> 2349226

Integration of human immunodeficiency virus type 1 DNA in vitro.

C M Farnet1, W A Haseltine.   

Abstract

A highly efficient cell-free system for the integration of human immunodeficiency virus type 1 DNA is described. Linear viral DNA synthesis occurs in the cytoplasm of newly infected cells, reaching peak levels 4 hr after infection. The linear viral DNA molecules present in cytoplasmic extracts are capable of integrating into heterologous DNA targets in vitro. The viral DNA resides in a high molecular weight nucleoprotein structure that can be separated from the bulk of cellular protein and nucleic acid without a detectable decrease in the ability to integrate in vitro.

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Year:  1990        PMID： 2349226      PMCID： PMC54068          DOI： 10.1073/pnas.87.11.4164

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  6 in total

1.  Retroviral integration: structure of the initial covalent product and its precursor, and a role for the viral IN protein.

Authors:  P O Brown; B Bowerman; H E Varmus; J M Bishop
Journal:  Proc Natl Acad Sci U S A       Date:  1989-04       Impact factor: 11.205

2.  Correct integration of retroviral DNA in vitro.

Authors:  P O Brown; B Bowerman; H E Varmus; J M Bishop
Journal:  Cell       Date:  1987-05-08       Impact factor: 41.582

3.  A nucleoprotein complex mediates the integration of retroviral DNA.

Authors:  B Bowerman; P O Brown; J M Bishop; H E Varmus
Journal:  Genes Dev       Date:  1989-04       Impact factor: 11.361

4.  Adaptation of the bicinchoninic acid protein assay for use with microtiter plates and sucrose gradient fractions.

Authors:  M G Redinbaugh; R B Turley
Journal:  Anal Biochem       Date:  1986-03       Impact factor: 3.365

5.  Temporal aspects of DNA and RNA synthesis during human immunodeficiency virus infection: evidence for differential gene expression.

Authors:  S Y Kim; R Byrn; J Groopman; D Baltimore
Journal:  J Virol       Date:  1989-09       Impact factor: 5.103

6.  Retroviral DNA integration: structure of an integration intermediate.

Authors:  T Fujiwara; K Mizuuchi
Journal:  Cell       Date:  1988-08-12       Impact factor: 41.582
  6 in total
  126 in total

Review 1.  Retroviral DNA integration.

Authors:  P Hindmarsh; J Leis
Journal:  Microbiol Mol Biol Rev       Date:  1999-12       Impact factor: 11.056

2.  The C-terminal proline-rich tail of human immunodeficiency virus type 2 Vpx is necessary for nuclear localization of the viral preintegration complex in nondividing cells.

Authors:  H A Pancio; N Vander Heyden; L Ratner
Journal:  J Virol       Date:  2000-07       Impact factor: 5.103

3.  Retroviral cDNA integration: stimulation by HMG I family proteins.

Authors:  L Li; K Yoder; M S Hansen; J Olvera; M D Miller; F D Bushman
Journal:  J Virol       Date:  2000-12       Impact factor: 5.103

4.  Asymmetric processing of human immunodeficiency virus type 1 cDNA in vivo: implications for functional end coupling during the chemical steps of DNA transposition.

Authors:  H Chen; A Engelman
Journal:  Mol Cell Biol       Date:  2001-10       Impact factor: 4.272

5.  Role of the non-homologous DNA end joining pathway in the early steps of retroviral infection.

Authors:  L Li; J M Olvera; K E Yoder; R S Mitchell; S L Butler; M Lieber; S L Martin; F D Bushman
Journal:  EMBO J       Date:  2001-06-15       Impact factor: 11.598

6.  Characterization of a replication-defective human immunodeficiency virus type 1 att site mutant that is blocked after the 3' processing step of retroviral integration.

Authors:  H Chen; A Engelman
Journal:  J Virol       Date:  2000-09       Impact factor: 5.103

7.  Characterization of chemokine receptor utilization of viruses in the latent reservoir for human immunodeficiency virus type 1.

Authors:  T Pierson; T L Hoffman; J Blankson; D Finzi; K Chadwick; J B Margolick; C Buck; J D Siliciano; R W Doms; R F Siliciano
Journal:  J Virol       Date:  2000-09       Impact factor: 5.103

8.  Integrase-lexA fusion proteins incorporated into human immunodeficiency virus type 1 that contains a catalytically inactive integrase gene are functional to mediate integration.

Authors:  M L Holmes-Son; S A Chow
Journal:  J Virol       Date:  2000-12       Impact factor: 5.103

9.  Quantitative analysis of HIV-1 preintegration complexes.

Authors:  Alan Engelman; Ilker Oztop; Nick Vandegraaff; Nidhanapati K Raghavendra
Journal:  Methods       Date:  2009-02-20       Impact factor: 3.608

10.  Human immunodeficiency virus type 1 integrase: effect on viral replication of mutations at highly conserved residues.

Authors:  P M Cannon; W Wilson; E Byles; S M Kingsman; A J Kingsman
Journal:  J Virol       Date:  1994-08       Impact factor: 5.103
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