Literature DB >> 7635840

Expression of a functional fungal polyketide synthase in the bacterium Streptomyces coelicolor A3(2).

D J Bedford1, E Schweizer, D A Hopwood, C Khosla.   

Abstract

The multifunctional 6-methylsalicylic acid synthase gene from Penicillium patulum was engineered for regulated expression in Streptomyces coelicolor. Production of significant amounts of 6-methylsalicylic acid by the recombinant strain was proven by nuclear magnetic resonance spectroscopy. These results suggest that it is possible to harness the molecular diversity of eukaryotic polyketide pathways by heterologous expression of biosynthetic genes in an easily manipulated model bacterial host in which prokaryotic aromatic and modular polyketide synthase genes are already expressed and recombined.

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Year:  1995        PMID： 7635840      PMCID： PMC177212          DOI： 10.1128/jb.177.15.4544-4548.1995

Source DB:  PubMed          Journal:  J Bacteriol        ISSN： 0021-9193            Impact factor:   3.490


  26 in total

1.  An unusually large multifunctional polypeptide in the erythromycin-producing polyketide synthase of Saccharopolyspora erythraea.

Authors:  J Cortes; S F Haydock; G A Roberts; D J Bevitt; P F Leadlay
Journal:  Nature       Date:  1990-11-08       Impact factor: 49.962

Review 2.  Molecular genetics of polyketides and its comparison to fatty acid biosynthesis.

Authors:  D A Hopwood; D H Sherman
Journal:  Annu Rev Genet       Date:  1990       Impact factor: 16.830

3.  Modular organization of genes required for complex polyketide biosynthesis.

Authors:  S Donadio; M J Staver; J B McAlpine; S J Swanson; L Katz
Journal:  Science       Date:  1991-05-03       Impact factor: 47.728

4.  The nucleotide sequence of a 16S rRNA gene from Streptomyces coelicolor A3(2)

Authors:  H A Baylis; M J Bibb
Journal:  Nucleic Acids Res       Date:  1987-09-11       Impact factor: 16.971

5.  [Biosynthesis of 6-methylsalicylic acid].

Authors:  P Dimroth; H Walter; F Lynen
Journal:  Eur J Biochem       Date:  1970-03-01

6.  The multifunctional 6-methylsalicylic acid synthase gene of Penicillium patulum. Its gene structure relative to that of other polyketide synthases.

Authors:  J Beck; S Ripka; A Siegner; E Schiltz; E Schweizer
Journal:  Eur J Biochem       Date:  1990-09-11

7.  Engineered biosynthesis of novel polyketides: evidence for temporal, but not regiospecific, control of cyclization of an aromatic polyketide precursor.

Authors:  H Fu; D A Hopwood; C Khosla
Journal:  Chem Biol       Date:  1994-12

8.  Characterization of a unique methyl-specific restriction system in Streptomyces avermitilis.

Authors:  D J MacNeil
Journal:  J Bacteriol       Date:  1988-12       Impact factor: 3.490

9.  Structure and deduced function of the granaticin-producing polyketide synthase gene cluster of Streptomyces violaceoruber Tü22.

Authors:  D H Sherman; F Malpartida; M J Bibb; H M Kieser; M J Bibb; D A Hopwood
Journal:  EMBO J       Date:  1989-09       Impact factor: 11.598

10.  Analysis of the nucleotide sequence of the Streptomyces glaucescens tcmI genes provides key information about the enzymology of polyketide antibiotic biosynthesis.

Authors:  M J Bibb; S Biró; H Motamedi; J F Collins; C R Hutchinson
Journal:  EMBO J       Date:  1989-09       Impact factor: 11.598
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  18 in total

Review 1.  Biosynthesis of sphinganine-analog mycotoxins.

Authors:  L Du; X Zhu; R Gerber; J Huffman; L Lou; J Jorgenson; F Yu; K Zaleta-Rivera; Q Wang
Journal:  J Ind Microbiol Biotechnol       Date:  2008-01-24       Impact factor: 3.346

2.  Functional replacement of the ketosynthase domain of FUM1 for the biosynthesis of fumonisins, a group of fungal reduced polyketides.

Authors:  X Zhu; F Yu; R S Bojja; K Zaleta-Rivera; L Du
Journal:  J Ind Microbiol Biotechnol       Date:  2006-05-09       Impact factor: 3.346

Review 3.  Biosynthesis of polyketides in heterologous hosts.

Authors:  B A Pfeifer; C Khosla
Journal:  Microbiol Mol Biol Rev       Date:  2001-03       Impact factor: 11.056

Review 4.  Metabolic engineering for the production of natural products.

Authors:  Lauren B Pickens; Yi Tang; Yit-Heng Chooi
Journal:  Annu Rev Chem Biomol Eng       Date:  2011       Impact factor: 11.059

5.  Production of 6-methylsalicylic acid by expression of a fungal polyketide synthase activates disease resistance in tobacco.

Authors:  N Yalpani; D J Altier; E Barbour; A L Cigan; C J Scelonge
Journal:  Plant Cell       Date:  2001-06       Impact factor: 11.277

Review 6.  Pseudomonas syringae phytotoxins: mode of action, regulation, and biosynthesis by peptide and polyketide synthetases.

Authors:  C L Bender; F Alarcón-Chaidez; D C Gross
Journal:  Microbiol Mol Biol Rev       Date:  1999-06       Impact factor: 11.056

7.  Antibiotic activity of polyketide products derived from combinatorial biosynthesis: implications for directed evolution.

Authors:  H Fu; C Khosla
Journal:  Mol Divers       Date:  1996-02       Impact factor: 2.943

8.  A gene (pks2) encoding a putative 6-methylsalicylic acid synthase from Glarea lozoyensis.

Authors:  P Lu; A Zhang; L M Dennis; A M Dahl-Roshak; Y-Q Xia; B Arison; Z An; J S Tkacz
Journal:  Mol Genet Genomics       Date:  2005-03-31       Impact factor: 3.291

9.  Polyketide synthase gene pksM from Aspergillus terreus expressed during growth phase.

Authors:  S Pazoutová; M Linka; S Storková; H Schwab
Journal:  Folia Microbiol (Praha)       Date:  1997       Impact factor: 2.099

10.  Production of a polyketide natural product in nonpolyketide-producing prokaryotic and eukaryotic hosts.

Authors:  J T Kealey; L Liu; D V Santi; M C Betlach; P J Barr
Journal:  Proc Natl Acad Sci U S A       Date:  1998-01-20       Impact factor: 11.205
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