Literature DB >> 762109

Purification and characterization of the sequence-specific endonuclease Bam HI.

L A Smith, J G Chirikjian.   

Abstract

The specific endonuclease Bam HI from Bacillus amyloliquefaciens (RUB 500) has been purified to apparent homogeneity. Two active forms of the enzyme corresponding to the dimeric and tetrameric forms have been isolated. On sodium dodecyl sulfate-polyacrylamide gel electrophoresis, the enzyme dissociated into Mr = 22,000 +/- 500 subunits. Bam HI has a broad pH optimum on the alkaline side and requires Mg2+ which can be partially replaced by Mn2+. The enzyme catalysis appears to be governed by a two-step mechanism.

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Year:  1979        PMID： 762109

Source DB:  PubMed          Journal:  J Biol Chem        ISSN： 0021-9258            Impact factor:   5.157


  10 in total

1.  Positive co-operative interaction between the subunits of CeqI restriction endonuclease.

Authors:  Z Jobbágy; Z Izsvák; E Duda
Journal:  Biochem J       Date:  1992-08-15       Impact factor: 3.857

2.  Characterization of the cloned BamHI restriction modification system: its nucleotide sequence, properties of the methylase, and expression in heterologous hosts.

Authors:  J E Brooks; P D Nathan; D Landry; L A Sznyter; P Waite-Rees; C L Ives; L S Moran; B E Slatko; J S Benner
Journal:  Nucleic Acids Res       Date:  1991-02-25       Impact factor: 16.971

3.  Cloning the BamHI restriction modification system.

Authors:  J E Brooks; J S Benner; D F Heiter; K R Silber; L A Sznyter; T Jager-Quinton; L S Moran; B E Slatko; G G Wilson; D O Nwankwo
Journal:  Nucleic Acids Res       Date:  1989-02-11       Impact factor: 16.971

4.  Physical and kinetic properties of the site specific endonuclease Bam HI from Bacillus amylolique-faciens.

Authors:  B Hinsch; M R Kula
Journal:  Nucleic Acids Res       Date:  1980-02-11       Impact factor: 16.971

5.  Sequence-specific endonuclease BamHI: relaxation of sequence recognition.

Authors:  J George; J G Chirikjian
Journal:  Proc Natl Acad Sci U S A       Date:  1982-04       Impact factor: 11.205

6.  BspRI restriction endonuclease: cloning, expression in Escherichia coli and sequential cleavage mechanism.

Authors:  Tamás Raskó; András Dér; Eva Klement; Krystyna Slaska-Kiss; Eszter Pósfai; Katalin F Medzihradszky; Daniel R Marshak; Richard J Roberts; Antal Kiss
Journal:  Nucleic Acids Res       Date:  2010-06-29       Impact factor: 16.971

7.  Regulation of the BamHI restriction-modification system by a small intergenic open reading frame, bamHIC, in both Escherichia coli and Bacillus subtilis.

Authors:  C L Ives; P D Nathan; J E Brooks
Journal:  J Bacteriol       Date:  1992-11       Impact factor: 3.490

8.  Cofactor requirements of BamHI mutant endonuclease E77K and its suppressor mutants.

Authors:  S Y Xu; I Schildkraut
Journal:  J Bacteriol       Date:  1991-08       Impact factor: 3.490

9.  Cloning of a restriction-modification system from Proteus vulgaris and its use in analyzing a methylase-sensitive phenotype in Escherichia coli.

Authors:  R M Blumenthal; S A Gregory; J S Cooperider
Journal:  J Bacteriol       Date:  1985-11       Impact factor: 3.490

10.  Synthesis and activity of three new trinuclear platinums with cis-geometry for terminal metal centres.

Authors:  Shahnaz A Hamad; Philip Beale; Jun Q Yu; Fazlul Huq
Journal:  J Biomed Sci       Date:  2014-05-12       Impact factor: 8.410
  10 in total

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