Literature DB >> 6255448

Physical and kinetic properties of the site specific endonuclease Bam HI from Bacillus amylolique-faciens.

B Hinsch, M R Kula.   

Abstract

The site specific endonuclease Bam HI which is composed of subunits of a molecular weight of 22 000 [1] can aggregate to complexes of a molecular weight of 360 000. It is an acidic protein with an isoelectric point at pH 5.3. Optimal activity is reached at 13 mM MgCl2. A very simple method is presented to determine kinetic constants of restriction enzymes directly from agarose gel photographs without any further equipment applying the integrated Michaelis Menten equation. With pJC 80 DNA as a substrate KM was found to be 3.6 10(-10) M. The method can be used to redefine the unit activity of site specific endonucleases unambigously.

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Year:  1980        PMID： 6255448      PMCID： PMC327295          DOI： 10.1093/nar/8.3.623

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  13 in total

1.  Studies on the cleavage of bacteriophage lambda DNA with EcoRI Restriction endonuclease.

Authors:  M Thomas; R W Davis
Journal:  J Mol Biol       Date:  1975-01-25       Impact factor: 5.469

2.  Class of small multicopy plasmids originating from the mutant antibiotic resistance factor R1 drd-19B2.

Authors:  W Goebel; R Bonewald
Journal:  J Bacteriol       Date:  1975-08       Impact factor: 3.490

3.  Isolation and properties of a thermostable restriction endonuclease (ENDO R-Bst1503).

Authors:  J F Catterall; N E Welker
Journal:  J Bacteriol       Date:  1977-02       Impact factor: 3.490

4.  Substrate dependence of the mechanism of EcoRI endonuclease.

Authors:  R A Rubin; P Modrich
Journal:  Nucleic Acids Res       Date:  1978-08       Impact factor: 16.971

5.  Restriction endonucleases: a new role in vivo?

Authors:  R J Roberts
Journal:  Nature       Date:  1978-02-09       Impact factor: 49.962

6.  Association kinetics with coupled diffusion. An extension to coiled-chain macromolecules applied to the lac repressor-operator system.

Authors:  O G Berg; C Blomberg
Journal:  Biophys Chem       Date:  1977-06       Impact factor: 2.352

7.  Detection of two restriction endonuclease activities in Haemophilus parainfluenzae using analytical agarose--ethidium bromide electrophoresis.

Authors:  P A Sharp; B Sugden; J Sambrook
Journal:  Biochemistry       Date:  1973-07-31       Impact factor: 3.162

8.  Recognition sequence of specific endonuclease BamH.I from Bacillus amyloliquefaciens H.

Authors:  R J Roberts; G A Wilson; F E Young
Journal:  Nature       Date:  1977-01-06       Impact factor: 49.962

9.  EcoRI endonuclease. Physical and catalytic properties of the homogenous enzyme.

Authors:  P Modrich; D Zabel
Journal:  J Biol Chem       Date:  1976-10-10       Impact factor: 5.157

10.  Purification and characterization of the sequence-specific endonuclease Bam HI.

Authors:  L A Smith; J G Chirikjian
Journal:  J Biol Chem       Date:  1979-02-25       Impact factor: 5.157
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  4 in total

1.  A continuous assay for DNA cleavage: the application of "break lights" to enediynes, iron-dependent agents, and nucleases.

Authors:  J B Biggins; J R Prudent; D J Marshall; M Ruppen; J S Thorson
Journal:  Proc Natl Acad Sci U S A       Date:  2000-12-05       Impact factor: 11.205

Review 2.  Type II restriction endonucleases--a historical perspective and more.

Authors:  Alfred Pingoud; Geoffrey G Wilson; Wolfgang Wende
Journal:  Nucleic Acids Res       Date:  2014-05-30       Impact factor: 16.971

3.  Binding of non-substrate nucleotides to a restriction endonuclease: a model for the interaction of bam HI with its recognition sequence.

Authors:  B Hinsch; H Mayer; M R Kula
Journal:  Nucleic Acids Res       Date:  1980-06-11       Impact factor: 16.971

4.  Reaction kinetics of some important site-specific endonucleases.

Authors:  B Hinsch; M R Kula
Journal:  Nucleic Acids Res       Date:  1981-07-10       Impact factor: 16.971
  4 in total

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