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Literature DB >> 7615753

Stability of free and complexed human immunodeficiency virus type 1 antigen at 4 degrees C and at room temperature.

Abstract

Free and immune-complex-dissociated (ICD) human immunodeficiency virus type 1 (HIV-1) antigenemias in serum specimens stored at room temperature (RT) and 4 degrees C for 1 to 35 days were evaluated. At all time points examined, there was no significant loss in detectable levels of ICD HIV-1 antigen at either RT or 4 degrees C. Free HIV-1 antigen was not stable in serum samples stored at RT for more than 2 days but was stable in samples stored at 4 degrees C for up to 4 days. Loss of free antigen occurred more rapidly in samples with high antigen content at baseline. Use of the ICD antigen assay allowed accurate quantitation of antigen in samples stored at RT or 4 degrees C for as long as 1 month.

Entities: Disease Species

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Year: 1995 PMID： 7615753 PMCID： PMC228161 DOI： 10.1128/jcm.33.5.1348-1350.1995

Source DB: PubMed Journal: J Clin Microbiol ISSN： 0095-1137 Impact factor: 5.948

18 in total

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Authors: M Portera; F Vitale; R La Licata; D R Alesi; G Lupo; F Bonura; N Romano; G Di Cuonzo
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Authors: P Nishanian; K R Huskins; S Stehn; R Detels; J L Fahey
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5. Acid hydrolysis of serum samples to increase detection of HIV antigen.

Authors: T Mathiesen; V A Sundqvist; J Albert; E Ohlsson; B Wahren
Journal: J Virol Methods Date: 1988-12 Impact factor: 2.014

6. Numbers of CD4+ cells and the levels of core antigens of and antibodies to the human immunodeficiency virus as predictors of AIDS among seropositive homosexual men.

Authors: F de Wolf; J M Lange; J T Houweling; R A Coutinho; P T Schellekens; J van der Noordaa; J Goudsmit
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7. Sensitive detection and early prognostic significance of p24 antigen in heat-denatured plasma of human immunodeficiency virus type 1-infected infants. Swiss Neonatal HIV Study Group.

Authors: J Schüpbach; J Böni; Z Tomasik; J Jendis; R Seger; C Kind
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Authors: T M McHugh; D P Stites; M P Busch; J F Krowka; R B Stricker; H Hollander
Journal: J Infect Dis Date: 1988-11 Impact factor: 5.226

9. The antiviral effect of zidovudine and ribavirin in clinical trials and the use of p24 antigen levels as a virologic marker.

Authors: S A Spector; C Kennedy; J A McCutchan; S A Bozzette; R G Straube; J D Connor; D D Richman
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