| Literature DB >> 3220923 |
T Mathiesen1, V A Sundqvist, J Albert, E Ohlsson, B Wahren.
Abstract
Sera from 32 HIV-infected patients and 20 controls were assayed for HIV antigen (HIV-Ag) and antibodies following acid hydrolysis. Acid hydrolysis, followed by neutralization immediately prior to the assay, was found to be a simple means to solubilize immune complexes and allowed recovery of 40-50% of complexed antigen. Following acid hydrolysis, HIV-Ag levels rose or became detectable in 22/32 patients and HIV IgG1-4 levels rose in 17/20 patients studied. The results show that complexed HIV-Ag may evade detection in HIV-infected patients.Entities:
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Year: 1988 PMID： 3220923 DOI： 10.1016/0166-0934(88)90097-3
Source DB: PubMed Journal: J Virol Methods ISSN： 0166-0934 Impact factor: 2.014