OBJECTIVE: To investigate the use of intracytoplasmic sperm injection with sperm collected by fine needle biopsy of the testis as a treatment for male genital tract obstruction. DESIGN: Sperm isolated from a fine needle biopsy of the testis were used to inseminate oocytes by intracytoplasmic sperm injection. SETTING: A hospital-based tertiary referral infertility service. PATIENTS: Case studies of two couples in whom the male partner had a genital tract obstruction. MAIN OUTCOME MEASURES: Fertilization and pregnancy. RESULTS: In the first case, 9 oocytes fertilized normally out of 13 injected. After the transfer of fresh (one cycle) and frozen (four cycles) embryos, a single intrauterine fetal heart pregnancy was achieved. In the second case, five oocytes fertilized normally from nine oocytes injected; two embryos were transferred fresh and three were frozen. A single fetal heart intrauterine gestation was obtained after the initial transfer of two fresh embryos. CONCLUSIONS: A high normal fertilization rate and pregnancies are possible with intracytoplasmic sperm injection using sperm collected directly from the testis. Sperm retrieval by fine needle biopsy offers a viable alternative to microsurgical aspiration and is also suitable for treating patients with intratesticular blockage.
OBJECTIVE: To investigate the use of intracytoplasmic sperm injection with sperm collected by fine needle biopsy of the testis as a treatment for male genital tract obstruction. DESIGN: Sperm isolated from a fine needle biopsy of the testis were used to inseminate oocytes by intracytoplasmic sperm injection. SETTING: A hospital-based tertiary referral infertility service. PATIENTS: Case studies of two couples in whom the male partner had a genital tract obstruction. MAIN OUTCOME MEASURES: Fertilization and pregnancy. RESULTS: In the first case, 9 oocytes fertilized normally out of 13 injected. After the transfer of fresh (one cycle) and frozen (four cycles) embryos, a single intrauterine fetal heart pregnancy was achieved. In the second case, five oocytes fertilized normally from nine oocytes injected; two embryos were transferred fresh and three were frozen. A single fetal heart intrauterine gestation was obtained after the initial transfer of two fresh embryos. CONCLUSIONS: A high normal fertilization rate and pregnancies are possible with intracytoplasmic sperm injection using sperm collected directly from the testis. Sperm retrieval by fine needle biopsy offers a viable alternative to microsurgical aspiration and is also suitable for treating patients with intratesticular blockage.
Authors: Jitske Eliveld; Madelon van Wely; Andreas Meißner; Sjoerd Repping; Fulco van der Veen; Ans M M van Pelt Journal: Hum Reprod Update Date: 2018-07-01 Impact factor: 15.610