Literature DB >> 7606186

Evaluation of random amplified polymorphic DNA (RAPD)-PCR as a method to differentiate Lactobacillus acidophilus, Lactobacillus crispatus, Lactobacillus amylovorus, Lactobacillus gallinarum, Lactobacillus gasseri, and Lactobacillus johnsonii.

E M Du Plessis1, L M Dicks.   

Abstract

The technique random amplified polymorphic DNA (RAPD)-PCR was evaluated as a method to differentiate Lactobacillus acidophilus, Lactobacillus crispatus, Lactobacillus amylovorus, Lactobacillus gallinarum, Lactobacillus gasseri, and Lactobacillus johnsonii. Representative strains, including the type of each species, were selected from different clusters obtained by numerical analysis of total soluble cell protein patterns. Results obtained by RAPD-PCR corresponded well with results obtained by numerical analysis of total soluble cell protein patterns. The type strains of each species displayed different RAPD profiles. Strains with identical L(+)- nicotinamide adenine dinucleotide-dependent lactic dehydrogenase (nLDH) electrophoretic profiles could be distinguished on the basis of their RAPD profiles.

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Year:  1995        PMID： 7606186     DOI： 10.1007/BF00294286

Source DB:  PubMed          Journal:  Curr Microbiol        ISSN： 0343-8651            Impact factor:   2.188


  4 in total

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Authors:  K Kersters; J De Ley
Journal:  J Gen Microbiol       Date:  1975-04

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Authors:  F Dellaglio; V Bottazzi; L D Trovatelli
Journal:  J Gen Microbiol       Date:  1973-02

3.  Genetic heterogeneity among Lactobacillus acidophilus strains.

Authors:  P G Sarra; M Magri; V Bottazzi; F Dellaglio
Journal:  Antonie Van Leeuwenhoek       Date:  1980       Impact factor: 2.271

4.  Taxonomic study of the Lactobacillus acidophilus group, with recognition of Lactobacillus gallinarum sp. nov. and Lactobacillus johnsonii sp. nov. and synonymy of Lactobacillus acidophilus group A3 (Johnson et al. 1980) with the type strain of Lactobacillus amylovorus (Nakamura 1981).

Authors:  T Fujisawa; Y Benno; T Yaeshima; T Mitsuoka
Journal:  Int J Syst Bacteriol       Date:  1992-07
  4 in total
  13 in total

1.  Comparison of statistical methods for identification of Streptococcus thermophilus, Enterococcus faecalis, and Enterococcus faecium from randomly amplified polymorphic DNA patterns.

Authors:  G Moschetti; G Blaiotta; F Villani; S Coppola; E Parente
Journal:  Appl Environ Microbiol       Date:  2001-05       Impact factor: 4.792

Review 2.  Understanding vaginal microbiome complexity from an ecological perspective.

Authors:  Roxana J Hickey; Xia Zhou; Jacob D Pierson; Jacques Ravel; Larry J Forney
Journal:  Transl Res       Date:  2012-03-06       Impact factor: 7.012

Review 3.  The vaginal microbiome: new information about genital tract flora using molecular based techniques.

Authors:  R F Lamont; J D Sobel; R A Akins; S S Hassan; T Chaiworapongsa; J P Kusanovic; R Romero
Journal:  BJOG       Date:  2011-01-20       Impact factor: 6.531

4.  Characterization of a novel Lactobacillus species closely related to Lactobacillus johnsonii using a combination of molecular and comparative genomics methods.

Authors:  Luz-Adriana Sarmiento-Rubiano; Bernard Berger; Déborah Moine; Manuel Zúñiga; Gaspar Pérez-Martínez; María J Yebra
Journal:  BMC Genomics       Date:  2010-09-17       Impact factor: 3.969

5.  Peptidoglycan hydrolases as species-specific markers to differentiate Lactobacillus helveticus from Lactobacillus gallinarum and other closely related homofermentative lactobacilli.

Authors:  Iva Jebava; Victoria Chuat; Sylvie Lortal; Florence Valence
Journal:  Curr Microbiol       Date:  2013-12-21       Impact factor: 2.188

6.  Strain-specific identification of probiotic Lactobacillus rhamnosus with randomly amplified polymorphic DNA-derived PCR primers.

Authors:  A Tilsala-Timisjärvi; T Alatossava
Journal:  Appl Environ Microbiol       Date:  1998-12       Impact factor: 4.792

7.  DNA fingerprinting of lactic acid bacteria in sauerkraut fermentations.

Authors:  Vethachai Plengvidhya; Fredrick Breidt; Zhongjing Lu; Henry P Fleming
Journal:  Appl Environ Microbiol       Date:  2007-10-05       Impact factor: 4.792

8.  Identification and H(2)O(2) production of vaginal lactobacilli from pregnant women at high risk of preterm birth and relation with outcome.

Authors:  Mark Wilks; Rebecca Wiggins; Angela Whiley; Enid Hennessy; Simon Warwick; Helen Porter; Anthony Corfield; Michael Millar
Journal:  J Clin Microbiol       Date:  2004-02       Impact factor: 5.948

9.  Detection and identification of Lactobacillus species in crops of broilers of different ages by using PCR-denaturing gradient gel electrophoresis and amplified ribosomal DNA restriction analysis.

Authors:  Le Luo Guan; Karen E Hagen; Gerald W Tannock; Doug R Korver; Gaylene M Fasenko; Gwen E Allison
Journal:  Appl Environ Microbiol       Date:  2003-11       Impact factor: 4.792

10.  Development of a tiered multilocus sequence typing scheme for members of the Lactobacillus acidophilus complex.

Authors:  Padmini Ramachandran; David W Lacher; Erika A Pfeiler; Christopher A Elkins
Journal:  Appl Environ Microbiol       Date:  2013-09-13       Impact factor: 4.792
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