Literature DB >> 7597109

A Tn4001 delivery system for Streptococcus gordonii (Challis).

R D Lunsford1.   

Abstract

The Staphylococcus aureus transposon Tn4001 was found to transpose in Streptococcus gordonii. Transposition sites appeared to be randomly distributed, and the element was stable in the absence of antibiotic selection. An increase in transposition frequency was noted when the delivery plasmid was propagated in a Dam- Dcm- Escherichia coli host strain. The utility of Tn4001 was demonstrated by the generation of lactose-negative mutants. Small size, clonal stability, random transposition, and known nucleotide sequence make Tn4001 a useful addition to the repertoire of tools available to the streptococcal geneticist.

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Year:  1995        PMID： 7597109     DOI： 10.1006/plas.1995.1016

Source DB:  PubMed          Journal:  Plasmid        ISSN： 0147-619X            Impact factor:   3.466


  14 in total

1.  Diversity of Tn4001 transposition products: the flanking IS256 elements can form tandem dimers and IS circles.

Authors:  M Prudhomme; C Turlan; J-P Claverys; M Chandler
Journal:  J Bacteriol       Date:  2002-01       Impact factor: 3.490

2.  Defects in D-alanyl-lipoteichoic acid synthesis in Streptococcus mutans results in acid sensitivity.

Authors:  D A Boyd; D G Cvitkovitch; A S Bleiweis; M Y Kiriukhin; D V Debabov; F C Neuhaus; I R Hamilton
Journal:  J Bacteriol       Date:  2000-11       Impact factor: 3.490

3.  Insertional inactivation of genes responsible for the D-alanylation of lipoteichoic acid in Streptococcus gordonii DL1 (Challis) affects intrageneric coaggregations.

Authors:  D L Clemans; P E Kolenbrander; D V Debabov; Q Zhang; R D Lunsford; H Sakone; C J Whittaker; M P Heaton; F C Neuhaus
Journal:  Infect Immun       Date:  1999-05       Impact factor: 3.441

4.  Identification of the operon for the sorbitol (Glucitol) Phosphoenolpyruvate:Sugar phosphotransferase system in Streptococcus mutans.

Authors:  D A Boyd; T Thevenot; M Gumbmann; A L Honeyman; I R Hamilton
Journal:  Infect Immun       Date:  2000-02       Impact factor: 3.441

5.  A role for trigger factor and an rgg-like regulator in the transcription, secretion and processing of the cysteine proteinase of Streptococcus pyogenes.

Authors:  W R Lyon; C M Gibson; M G Caparon
Journal:  EMBO J       Date:  1998-11-02       Impact factor: 11.598

6.  Insertional inactivation of an intrageneric coaggregation-relevant adhesin locus from Streptococcus gordonii DL1 (Challis).

Authors:  C J Whittaker; D L Clemans; P E Kolenbrander
Journal:  Infect Immun       Date:  1996-10       Impact factor: 3.441

7.  Formation of potent hybrid promoters of the mutant llm gene by IS256 transposition in methicillin-resistant Staphylococcus aureus.

Authors:  H Maki; K Murakami
Journal:  J Bacteriol       Date:  1997-11       Impact factor: 3.490

8.  Insertional mutagenesis and recovery of interrupted genes of Streptococcus mutans by using transposon Tn917: preliminary characterization of mutants displaying acid sensitivity and nutritional requirements.

Authors:  J A Gutierrez; P J Crowley; D P Brown; J D Hillman; P Youngman; A S Bleiweis
Journal:  J Bacteriol       Date:  1996-07       Impact factor: 3.490

9.  Visualizing pneumococcal infections in the lungs of live mice using bioluminescent Streptococcus pneumoniae transformed with a novel gram-positive lux transposon.

Authors:  K P Francis; J Yu; C Bellinger-Kawahara; D Joh; M J Hawkinson; G Xiao; T F Purchio; M G Caparon; M Lipsitch; P R Contag
Journal:  Infect Immun       Date:  2001-05       Impact factor: 3.441

10.  Natural genetic transformation in Streptococcus gordonii: comX imparts spontaneous competence on strain wicky.

Authors:  R D Lunsford; J London
Journal:  J Bacteriol       Date:  1996-10       Impact factor: 3.490
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