Literature DB >> 7592400

Instability of Rickettsia prowazekii RNA polymerase-promoter complexes.

L P Aniskovitch1, H H Winkler.   

Abstract

The Rickettsia prowazekii sigma factor was overexpressed, purified, and used to reconstitute RNA polymerase holoenzyme species. R. prowazekii RNA polymerase-promoter complexes were unstable and remained dissociable and heparin sensitive under conditions in which the corresponding Escherichia coli complexes were not. The R. prowazekii core played the major role in determining heparin sensitivity.

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Year:  1995        PMID： 7592400      PMCID： PMC177475          DOI： 10.1128/jb.177.21.6301-6303.1995

Source DB:  PubMed          Journal:  J Bacteriol        ISSN： 0021-9193            Impact factor:   3.490


  10 in total

1.  Factor-independent activation of Escherichia coli rRNA transcription. I. Kinetic analysis of the roles of the upstream activator region and supercoiling on transcription of the rrnB P1 promoter in vitro.

Authors:  S Leirmo; R L Gourse
Journal:  J Mol Biol       Date:  1991-08-05       Impact factor: 5.469

2.  Functional characteristics of the rrnD promoters of Escherichia coli.

Authors:  W Langert; M Meuthen; K Mueller
Journal:  J Biol Chem       Date:  1991-11-15       Impact factor: 5.157

3.  Visualization and quantitative analysis of complex formation between E. coli RNA polymerase and an rRNA promoter in vitro.

Authors:  R L Gourse
Journal:  Nucleic Acids Res       Date:  1988-10-25       Impact factor: 16.971

4.  Two modes of transcription initiation in vitro at the rrnB P1 promoter of Escherichia coli.

Authors:  S Borukhov; V Sagitov; C A Josaitis; R L Gourse; A Goldfarb
Journal:  J Biol Chem       Date:  1993-11-05       Impact factor: 5.157

5.  The main early and late promoters of Bacillus subtilis phage phi 29 form unstable open complexes with sigma A-RNA polymerase that are stabilized by DNA supercoiling.

Authors:  F Rojo; B Nuez; M Mencía; M Salas
Journal:  Nucleic Acids Res       Date:  1993-02-25       Impact factor: 16.971

6.  Purification and partial characterization of the DNA-dependent RNA polymerase from Rickettsia prowazekii.

Authors:  H F Ding; H H Winkler
Journal:  J Bacteriol       Date:  1990-10       Impact factor: 3.490

7.  The molar ratio of sigma 73 to core polymerase in the obligate intracellular bacterium, Rickettsia prowazekii.

Authors:  H F Ding; H H Winkler
Journal:  Mol Microbiol       Date:  1994-03       Impact factor: 3.501

8.  Isolation and characterization of the gene coding for the major sigma factor of Rickettsia prowazekii DNA-dependent RNA polymerase.

Authors:  G L Marks; H H Winkler; D O Wood
Journal:  Gene       Date:  1992-11-02       Impact factor: 3.688

9.  Characterization of the DNA-melting function of the Rickettsia prowazekii RNA polymerase.

Authors:  H F Ding; H H Winkler
Journal:  J Biol Chem       Date:  1993-02-25       Impact factor: 5.157

10.  Mechanism of initiation of transcription by Bacillus subtilis RNA polymerase at several promoters.

Authors:  F W Whipple; A L Sonenshein
Journal:  J Mol Biol       Date:  1992-01-20       Impact factor: 5.469
  10 in total
  3 in total

1.  Lineage-specific amino acid substitutions in region 2 of the RNA polymerase sigma subunit affect the temperature of promoter opening.

Authors:  N Barinova; E Zhilina; I Bass; V Nikiforov; A Kulbachinskiy
Journal:  J Bacteriol       Date:  2008-02-15       Impact factor: 3.490

2.  Transcriptional characterization of the Rickettsia prowazekii major macromolecular synthesis operon.

Authors:  E I Shaw; G L Marks; H H Winkler; D O Wood
Journal:  J Bacteriol       Date:  1997-10       Impact factor: 3.490

3.  Structural modules of RNA polymerase required for transcription from promoters containing downstream basal promoter element GGGA.

Authors:  Nataliya Barinova; Konstantin Kuznedelov; Konstantin Severinov; Andrey Kulbachinskiy
Journal:  J Biol Chem       Date:  2008-06-23       Impact factor: 5.157
  3 in total

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