Some factors affecting the airborne survival of bacteria outdoors.

Airborne survival of two pseudomonads and a reference strain of Escherichia coli (strain MRE 162) was studied outdoors using a modified microthread technique. When cells of E. coli were suspended as clusters, survival was much greater than single cells, particularly outdoors. Culture age had a highly significant effect on survival of Pseudomonas maltophila with survival of 24 h cultures being more than 100-fold higher than 48 h cultures. Survival of Pseudomonas fluorescens was variable and depended also upon the method of culture. Survival of E. coli and Ps. maltophila was studied at three locations differing in air quality and was found to be significantly reduced outdoors, particularly when held in direct daylight. Outdoor survival was not significantly different at the three locations but was reduced at increasing temperatures. There was no apparent effect of wind direction or air quality. Results are discussed with reference to the release of genetically-modified micro-organisms.