Literature DB >> 75805

A serum-free medium for the Mishell-Dutton system.

M Burger.   

Abstract

A serum-free medium which provides high recoveries of plaque-forming cells in the primary immune response to sheep erythrocytes is described. In this medium the serum was replaced by heated fetuin and by excess of nonessential amino acids. Glycopeptides, obtained by excessive pronase treatment of fetuin were partially capable of replacing the nonhydrolyzed fetuin.

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Year:  1977        PMID: 75805     DOI: 10.1002/eji.1830071216

Source DB:  PubMed          Journal:  Eur J Immunol        ISSN: 0014-2980            Impact factor:   5.532


  4 in total

1.  An absolute requirement for 2-mercaptoethanol in the in vitro primary immune response in the absence of serum.

Authors:  M Burger
Journal:  Immunology       Date:  1979-07       Impact factor: 7.397

2.  Immunization in vitro and production of monoclonal antibodies specific to insoluble and weakly immunogenic proteins.

Authors:  J Van Ness; U K Laemmli; D E Pettijohn
Journal:  Proc Natl Acad Sci U S A       Date:  1984-12       Impact factor: 11.205

3.  Immunization of mouse spleen cell cultures in the absence of serum and its proteins using SiO2 and 2-mercaptoethanol.

Authors:  M Burger
Journal:  Immunology       Date:  1982-02       Impact factor: 7.397

4.  Optimization of the culture medium composition for the antibody response of mouse spleen cells.

Authors:  D Zhu; I Lefkovits
Journal:  In Vitro       Date:  1984-08
  4 in total

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