Literature DB >> 6083563

Immunization in vitro and production of monoclonal antibodies specific to insoluble and weakly immunogenic proteins.

J Van Ness, U K Laemmli, D E Pettijohn.   

Abstract

A procedure is described for immunizing in vitro and stimulating proliferation of specific B-cell lymphocytes. The method is applicable to production of monoclonal antibodies against proteins that are soluble only in denaturing solvents. An induction period is described in which antigen is presented to the B-cell population in the absence of serum. Also, antigen is coupled to mitogenic silica, which allows the effective presentation of both soluble and insoluble antigens. The results indicate hybridomas can be obtained that secrete IgMs directed against highly conserved or weakly immunogenic antigens.

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Year:  1984        PMID： 6083563      PMCID： PMC392260          DOI： 10.1073/pnas.81.24.7897

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  34 in total

Review 1.  Control of lymphocyte stimulation in vitro: "help' and "suppression' in the light of lymphoid population dynamics.

Authors:  S C Knight
Journal:  J Immunol Methods       Date:  1982       Impact factor: 2.303

2.  Specific attachment of nuclear-mitotic apparatus protein to metaphase chromosomes and mitotic spindle poles: possible function in nuclear reassembly.

Authors:  J Van Ness; D E Pettijohn
Journal:  J Mol Biol       Date:  1983-12-05       Impact factor: 5.469

Review 3.  Theory and methods for immunization in culture and monoclonal antibody production.

Authors:  C L Reading
Journal:  J Immunol Methods       Date:  1982-09-30       Impact factor: 2.303

Review 4.  Human T lymphocyte antigens as defined by monoclonal antibodies.

Authors:  B F Haynes
Journal:  Immunol Rev       Date:  1981       Impact factor: 12.988

5.  Preparation of monoclonal antibodies: strategies and procedures.

Authors:  G Galfrè; C Milstein
Journal:  Methods Enzymol       Date:  1981       Impact factor: 1.600

6.  Monoclonal antibodies to hypothalamic growth hormone-releasing factor with picomoles of antigen.

Authors:  R A Luben; P Brazeau; P Böhlen; R Guillemin
Journal:  Science       Date:  1982-11-26       Impact factor: 47.728

7.  Higher order metaphase chromosome structure: evidence for metalloprotein interactions.

Authors:  C D Lewis; U K Laemmli
Journal:  Cell       Date:  1982-05       Impact factor: 41.582

8.  Regulation of pokeweed mitogen-induced human B-cell differentiation: effects of hydrocortisone.

Authors:  W Knapp; R Berger; B Posch
Journal:  Immunopharmacology       Date:  1982-10

9.  In vivo and in vitro effects of 1,1-dimethylhydrazine on selected immune functions.

Authors:  M J Tarr; R G Olsen; D L Jacobs
Journal:  Immunopharmacology       Date:  1982-04

10.  Production of monoclonal antibodies against calmodulin by in vitro immunization of spleen cells.

Authors:  R L Pardue; R C Brady; G W Perry; J R Dedman
Journal:  J Cell Biol       Date:  1983-04       Impact factor: 10.539
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  2 in total

1.  Production of a monoclonal antibody against the Snow Mountain agent of gastroenteritis by in vitro immunization of murine spleen cells.

Authors:  J Treanor; R Dolin; H P Madore
Journal:  Proc Natl Acad Sci U S A       Date:  1988-05       Impact factor: 11.205

Review 2.  Hybridoma technology: new developments of practical interest.

Authors:  S R Samoilovich; C B Dugan; A J Macario
Journal:  J Immunol Methods       Date:  1987-08-03       Impact factor: 2.303
  2 in total

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