Literature DB >> 7579661

Gene fusion expression systems in Escherichia coli.

E R LaVallie1, J M McCoy.   

Abstract

In recent years, Escherichia coli gene fusion expression systems have circumvented many of the problems inherent in the use of this bacterium for the production of recombinant proteins. These systems also provide a powerful means for identifying peptides or proteins with desired binding specificities. Gene fusion technology continues to expand with the introduction of new fusion partners, purification and detection tags, cleavage reagents and ways to display peptides on the surface of bacteria.

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Year:  1995        PMID: 7579661     DOI: 10.1016/0958-1669(95)80083-2

Source DB:  PubMed          Journal:  Curr Opin Biotechnol        ISSN: 0958-1669            Impact factor:   9.740


  33 in total

1.  A novel method of affinity-purifying proteins using a bis-arsenical fluorescein.

Authors:  K S Thorn; N Naber; M Matuska; R D Vale; R Cooke
Journal:  Protein Sci       Date:  2000-02       Impact factor: 6.725

2.  In vivo immobilization of fusion proteins on bioplastics by the novel tag BioF.

Authors:  Cristina Moldes; Pedro García; José L García; María A Prieto
Journal:  Appl Environ Microbiol       Date:  2004-06       Impact factor: 4.792

3.  Expression of human proteins at the Southeast Collaboratory for Structural Genomics.

Authors:  Michael R Mayer; Tamara A Dailey; Clay M Baucom; Jill L Supernak; Michael C Grady; Harris E Hawk; Harry A Dailey
Journal:  J Struct Funct Genomics       Date:  2004

4.  Expression and Activity Analysis of Fructosyltransferase from Aspergillus oryzae.

Authors:  Lihong Guan; Liping Chen; Yongsen Chen; Nu Zhang; Yawei Han
Journal:  Protein J       Date:  2017-08       Impact factor: 2.371

5.  Conformational stability of pGEX-expressed Schistosoma japonicum glutathione S-transferase: a detoxification enzyme and fusion-protein affinity tag.

Authors:  W Kaplan; P Hüsler; H Klump; J Erhardt; N Sluis-Cremer; H Dirr
Journal:  Protein Sci       Date:  1997-02       Impact factor: 6.725

6.  Cysteine protease falcipain 1 in Plasmodium falciparum is biochemically distinct from its isozymes.

Authors:  S L Goh; L L Goh; T S Sim
Journal:  Parasitol Res       Date:  2005-07-23       Impact factor: 2.289

Review 7.  Strategies for achieving high-level expression of genes in Escherichia coli.

Authors:  S C Makrides
Journal:  Microbiol Rev       Date:  1996-09

8.  The importance of protein-protein interactions for optimising oxygen activity in photosystem II: reconstitution with a recombinant thioredoxin--manganese stabilising protein.

Authors:  A K Williamson; J R Liggins; W Hillier; T Wydrzynski
Journal:  Photosynth Res       Date:  2007-05-05       Impact factor: 3.573

9.  High-level production, solubilization and purification of synthetic human GPCR chemokine receptors CCR5, CCR3, CXCR4 and CX3CR1.

Authors:  Hui Ren; Daoyong Yu; Baosheng Ge; Brian Cook; Zhinan Xu; Shuguang Zhang
Journal:  PLoS One       Date:  2009-02-18       Impact factor: 3.240

10.  Production and characterization of a recombinant single-chain antibody against Hantaan virus envelop glycoprotein.

Authors:  Jie Yang; Rui Chen; Junxia Wei; Fanglin Zhang; Yong Zhang; Lintao Jia; Yan Yan; Wen Luo; Yunxin Cao; Libo Yao; Jifeng Sun; Zhikai Xu; Angang Yang
Journal:  Appl Microbiol Biotechnol       Date:  2009-12-10       Impact factor: 4.813

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