Literature DB >> 7575473

Expression, purification and characterization of recombinant human N-acetylgalactosamine-6-sulphatase.

J Bielicki1, M Fuller, X H Guo, C P Morris, J J Hopewood, D S Anson.   

Abstract

Full-length cDNA sequences encoding human N-acetylgalactosamine-6-sulphatase were stably expressed in Chinese hamster ovary cells under the transcriptional control of the human polypeptide chain elongation factor 1 alpha gene promoter. A clonal cell line overexpressing recombinant N-acetylgalactosamine-6-sulphatase to a level of approx. 3 mg/l of culture medium was isolated. The secreted precursor enzyme was purified to homogeneity by a two-column procedure with an overall yield of 53% of the activity. The physical and catalytic parameters of the recombinant enzyme were similar to those of the mature form isolated from liver. On SDS/PAGE and gel filtration, recombinant N-acetylgalactosamine-6-sulphatase had a native molecular mass of 58-60 kDa. Recombinant N-acetylgalactosamine-6-sulphatase was endocytosed by mucopolysaccharidosis IVA fibroblasts via the mannose-6-phosphate receptor-mediated pathway and was efficiently localized to lysosomes.

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Year:  1995        PMID： 7575473      PMCID： PMC1136156          DOI： 10.1042/bj3110333

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  19 in total

1.  Studies on glucosaminidase. 4. The fluorimetric assay of N-acetyl-beta-glucosaminidase.

Authors:  D H LEABACK; P G WALKER
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Authors:  J J Hopwood; H Elliott; V J Muller; G T Saccone
Journal:  Biochem J       Date:  1986-03-15       Impact factor: 3.857

3.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

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4.  Human liver iduronate-2-sulphatase. Purification, characterization and catalytic properties.

Authors:  J Bielicki; C Freeman; P R Clements; J J Hopwood
Journal:  Biochem J       Date:  1990-10-01       Impact factor: 3.857

5.  Molecular analysis by Southern blot for the N-acetylgalactosamine-6-sulphate sulphatase gene causing mucopolysaccharidosis IVA in the Japanese population.

Authors:  S Tomatsu; S Fukuda; A Uchiyama; T Hori; Y Nakashima; N Kondo; Y Suzuki; N Shimozawa; K Sukegawa; T Orii
Journal:  J Inherit Metab Dis       Date:  1994       Impact factor: 4.982

6.  Enzymatic diagnosis of the mucopolysaccharidoses: experience of 96 cases diagnosed in a five-year period.

Authors:  J J Hopwood; V Muller; J R Harrison; W F Carey; H Elliott; E F Robertson; A C Pollard
Journal:  Med J Aust       Date:  1982-03-20       Impact factor: 7.738

7.  Expression of a cloned human interleukin-2 cDNA is enhanced by the substitution of a heterologous mRNA leader region.

Authors:  B R Cullen
Journal:  DNA       Date:  1988-11

8.  Human alpha-L-iduronidase. 2. Catalytic properties.

Authors:  P R Clements; V Muller; J J Hopwood
Journal:  Eur J Biochem       Date:  1985-10-01

9.  Recombinant alpha-L-iduronidase: characterization of the purified enzyme and correction of mucopolysaccharidosis type I fibroblasts.

Authors:  E G Unger; J Durrant; D S Anson; J J Hopwood
Journal:  Biochem J       Date:  1994-11-15       Impact factor: 3.857

10.  Effect of ammonium chloride on subcellular distribution of lysosomal enzymes in human fibroblasts.

Authors:  P L Chang; M Ameen; C Z Yu; B M Kelly
Journal:  Exp Cell Res       Date:  1988-06       Impact factor: 3.905
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  15 in total

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3.  Enzyme replacement therapy for Morquio A: an active recombinant N-acetylgalactosamine-6-sulfate sulfatase produced in Escherichia coli BL21.

Authors:  Alexander Rodríguez; Angela J Espejo; Alejandra Hernández; Olga L Velásquez; Lina M Lizaraso; Henry A Cordoba; Oscar F Sánchez; Carlos J Alméciga-Díaz; Luis A Barrera
Journal:  J Ind Microbiol Biotechnol       Date:  2010-06-27       Impact factor: 3.346

4.  Recombinant human sulphamidase: expression, amplification, purification and characterization.

Authors:  J Bielicki; J J Hopwood; E L Melville; D S Anson
Journal:  Biochem J       Date:  1998-01-01       Impact factor: 3.857

5.  Expression, purification and characterization of recombinant caprine N-acetylglucosamine-6-sulphatase.

Authors:  T Litjens; J Bielicki; D S Anson; K Friderici; M Z Jones; J J Hopwood
Journal:  Biochem J       Date:  1997-10-01       Impact factor: 3.857

6.  Immortalization and characterization of a cell line exhibiting a severe multiple sulphatase deficiency phenotype.

Authors:  K Nelson; J Bielicki; D S Anson
Journal:  Biochem J       Date:  1997-08-15       Impact factor: 3.857

7.  Effect of elongation factor 1alpha promoter and SUMF1 over in vitro expression of N-acetylgalactosamine-6-sulfate sulfatase.

Authors:  Carlos J Alméciga-Díaz; Maria A Rueda-Paramo; Angela J Espejo; Olga Y Echeverri; Adriana Montaño; Shunji Tomatsu; Luis A Barrera
Journal:  Mol Biol Rep       Date:  2008-11-07       Impact factor: 2.316

8.  Cloning and characterization of a novel chondroitin sulfate/dermatan sulfate 4-O-endosulfatase from a marine bacterium.

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Journal:  J Biol Chem       Date:  2015-02-03       Impact factor: 5.157

9.  Overexpression of inactive arylsulphatase mutants and in vitro activation by light-dependent oxidation with vanadate.

Authors:  Terri M Christianson; Chris M Starr; Todd C Zankel
Journal:  Biochem J       Date:  2004-09-01       Impact factor: 3.857

10.  The structure of human GALNS reveals the molecular basis for mucopolysaccharidosis IV A.

Authors:  Yadilette Rivera-Colón; Emily K Schutsky; Adriana Z Kita; Scott C Garman
Journal:  J Mol Biol       Date:  2012-08-29       Impact factor: 5.469
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