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Literature DB >> 7574620

Direct detection of Listeria monocytogenes in 25 milliliters of raw milk by a two-step PCR with nested primers.

L M Herman¹, J H De Block, R J Moermans.

Abstract

A method for direct detection of Listeria monocytogenes in 25 ml of raw milk is presented. The detection limit can be situated between 10 and 5 CFU. The detection method is based on chemical extraction of the milk components and PCR amplification with two nested pairs of primers specific for Listeria monocytogenes.

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Year: 1995 PMID： 7574620 PMCID： PMC167343 DOI： 10.1128/aem.61.2.817-819.1995

Source DB: PubMed Journal: Appl Environ Microbiol ISSN： 0099-2240 Impact factor: 4.792

13 in total

1. Ultra sensitive detection of Listeria monocytogenes in milk by the polymerase chain reaction (PCR).

Authors: M A Starbuck; P J Hill; G S Stewart
Journal: Lett Appl Microbiol Date: 1992-12 Impact factor: 2.858

2. DNA amplification to enhance detection of genetically engineered bacteria in environmental samples.

Authors: R J Steffan; R M Atlas
Journal: Appl Environ Microbiol Date: 1988-09 Impact factor: 4.792

3. Detection of pathogenic Yersinia enterocolitica in foods and water by immunomagnetic separation, nested polymerase chain reactions, and colorimetric detection of amplified DNA.

Authors: G Kapperud; T Vardund; E Skjerve; E Hornes; T E Michaelsen
Journal: Appl Environ Microbiol Date: 1993-09 Impact factor: 4.792

4. Sensitive and specific detection of Listeria monocytogenes in milk and ground beef with the polymerase chain reaction.

Authors: E J Thomas; R K King; J Burchak; V P Gannon
Journal: Appl Environ Microbiol Date: 1991-09 Impact factor: 4.792

5. Rapid and sensitive method for the detection of serum hepatitis B virus DNA using the polymerase chain reaction technique.

Authors: S Kaneko; S M Feinstone; R H Miller
Journal: J Clin Microbiol Date: 1989-09 Impact factor: 5.948

6. Use of the polymerase chain reaction for direct detection of Listeria monocytogenes in soft cheese.

Authors: K Wernars; C J Heuvelman; T Chakraborty; S H Notermans
Journal: J Appl Bacteriol Date: 1991-02

7. Synthetic oligodeoxyribonucleotide probes for detection of Listeria monocytogenes.

Authors: A R Datta; B A Wentz; D Shook; M W Trucksess
Journal: Appl Environ Microbiol Date: 1988-12 Impact factor: 4.792

8. Detection of coliform bacteria in water by polymerase chain reaction and gene probes.

Authors: A K Bej; R J Steffan; J DiCesare; L Haff; R M Atlas
Journal: Appl Environ Microbiol Date: 1990-02 Impact factor: 4.792

9. Detection of Listeria species and Listeria monocytogenes using polymerase chain reaction.

Authors: P M Border; J J Howard; G S Plastow; K W Siggens
Journal: Lett Appl Microbiol Date: 1990-09 Impact factor: 2.858

10. Expression in Escherichia coli and sequence analysis of the listeriolysin O determinant of Listeria monocytogenes.

Authors: J Mengaud; M F Vicente; J Chenevert; J M Pereira; C Geoffroy; B Gicquel-Sanzey; F Baquero; J C Perez-Diaz; P Cossart
Journal: Infect Immun Date: 1988-04 Impact factor: 3.441

9 in total

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Authors: F Rossi; S Torriani; F Dellaglio
Journal: Appl Environ Microbiol Date: 1999-09 Impact factor: 4.792

Review 2. Methodologies for the characterization of microbes in industrial environments: a review.

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Journal: J Ind Microbiol Biotechnol Date: 2003-05-23 Impact factor: 3.346

3. Identification and detection of Bacillus sporothermodurans spores in 1, 10, and 100 milliliters of raw milk by PCR.

Authors: L M Herman; M J Vaerewijck; R J Moermans; G M Waes
Journal: Appl Environ Microbiol Date: 1997-08 Impact factor: 4.792

4. Development of a fluorogenic probe-based PCR assay for detection of Bacillus cereus in nonfat dry milk.

Authors: Y R Kim; J Czajka; C A Batt
Journal: Appl Environ Microbiol Date: 2000-04 Impact factor: 4.792

5. Immobilization with metal hydroxides as a means to concentrate food-borne bacteria for detection by cultural and molecular methods.

Authors: L A Lucore; M A Cullison; L A Jaykus
Journal: Appl Environ Microbiol Date: 2000-05 Impact factor: 4.792