Literature DB >> 7567

Neurospora crassa glutamine synthetase. Purification by affinity chromatography and characterization of subunit structure.

R Palacios.   

Abstract

Neurospora crassa glutamine synthetase was purified to homogeneity by a procedure based on affinity chromatography. The enzyme is adsorbed to a matrix of anthranilic acid bound to Sepharose and eluted with AMP. Different experimental approaches indicate that the enzyme has an octameric structure formed by subunits of identical molecular weight.

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Year:  1976        PMID： 7567

Source DB:  PubMed          Journal:  J Biol Chem        ISSN： 0021-9258            Impact factor:   5.157


  22 in total

1.  Heterogeneity of Glutamine Synthetase Polypeptides in Phaseolus vulgaris L.

Authors:  M Lara; H Porta; J Padilla; J Folch; F Sánchez
Journal:  Plant Physiol       Date:  1984-12       Impact factor: 8.340

2.  glnA mutations define the structural gene for glutamine synthetase in Aspergillus.

Authors:  E V Cornwell; D W Macdonald
Journal:  Curr Genet       Date:  1984-01       Impact factor: 3.886

3.  Genetics and physiology of Neurospora crassa glutamine auxotrophs.

Authors:  G Dávila; F Sánchez; R Palacios; J Mora
Journal:  J Bacteriol       Date:  1978-06       Impact factor: 3.490

4.  Nitrogen and ammonia assimilation in the cyanobacteria: purification of glutamine synthetase from Anabaena sp. strain CA.

Authors:  G Stacey; F R Tabita; C Van Baalen
Journal:  J Bacteriol       Date:  1977-11       Impact factor: 3.490

5.  Genetic and biochemical characterization of glutamine synthetase from Neurospora crassa glutamine auxotrophs and their revertants.

Authors:  G Dávila; S Brom; Y Mora; R Palacios; J Mora
Journal:  J Bacteriol       Date:  1983-12       Impact factor: 3.490

Review 6.  Regulation of nitrogen metabolism and gene expression in fungi.

Authors:  G A Marzluf
Journal:  Microbiol Rev       Date:  1981-09

7.  Effect of the gln-1b mutation on nitrogen metabolite repression in Neurospora crassa.

Authors:  N S Dunn-Coleman; R H Garrett
Journal:  J Bacteriol       Date:  1981-02       Impact factor: 3.490

8.  Purification and Characterization of Glutamine Synthetase and NADP-Glutamate Dehydrogenase from the Ectomycorrhizal Fungus Laccaria laccata.

Authors:  A Brun; M Chalot; B Botton; F Martin
Journal:  Plant Physiol       Date:  1992-07       Impact factor: 8.340

9.  Regulation of glutamine synthesis by glycine and serine in Neurospora crassa.

Authors:  G Hernández; Y Mora; J Mora
Journal:  J Bacteriol       Date:  1986-01       Impact factor: 3.490

10.  Physiology of ammonium assimilation in Neurospora crassa.

Authors:  M Lara; L Blanco; M Campomanes; E Calva; R Palacios; J Mora
Journal:  J Bacteriol       Date:  1982-04       Impact factor: 3.490
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