| Literature DB >> 7561147 |
B E Fayer1, P P Soni, M H Binger, D R Mould, H Satoh.
Abstract
A 'sandwich' enzyme-linked immunosorbent assay has been developed for measuring humanized anti-Tac (HAT), a humanized antibody to the IL-2 receptor on activated T cells (Tac), in human serum. The working range of this assay is 25-400 ng/ml with an overall precision of 5%. In this assay, the analyte, HAT, is sandwiched between Tac which is bound to a microtiter plate and biotinylated Tac that is conjugated to peroxidase labelled streptavidin. This assay was utilized to determine the pharmacokinetic parameters of HAT in patients with graft-versus-host disease.Entities:
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Year: 1995 PMID： 7561147 DOI： 10.1016/0022-1759(95)00131-s
Source DB: PubMed Journal: J Immunol Methods ISSN： 0022-1759 Impact factor: 2.303