Literature DB >> 7557480

Cloning and complete sequence of the DNA polymerase-encoding gene (BstpolI) and characterisation of the Klenow-like fragment from Bacillus stearothermophilus.

S M Phang1, C Y Teo, E Lo, V W Wong.   

Abstract

A fragment of the DNA polymerase I-encoding gene (polI) from Bacillus stearothermophilus (Bst) was obtained by PCR. This was used as a probe to obtain a full-length gene from a Bst genomic DNA (gDNA) plasmid library. Comparison of the sequence to B. caldotenax (Bca) showed about 93% homology at the amino acid (aa) level. A Klenow-like (BstpolIk) clone was developed and the recombinant protein displayed DNA polymerase activity similar to the wild-type BstPolI enzyme.

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Year:  1995        PMID： 7557480     DOI： 10.1016/0378-1119(95)00387-l

Source DB:  PubMed          Journal:  Gene        ISSN： 0378-1119            Impact factor:   3.688


  6 in total

1.  Preparation and Use of Cellular Reagents: A Low-resource Molecular Biology Reagent Platform.

Authors:  Sanchita Bhadra; Inyup Paik; Jose-Angel Torres; Stéphane Fadanka; Chiara Gandini; Harry Akligoh; Jenny Molloy; Andrew D Ellington
Journal:  Curr Protoc       Date:  2022-03

Review 2.  Loop-Mediated Isothermal Amplification Detection of SARS-CoV-2 and Myriad Other Applications.

Authors:  Keith J M Moore; Jeremy Cahill; Guy Aidelberg; Rachel Aronoff; Ali Bektaş; Daniela Bezdan; Daniel J Butler; Sridar V Chittur; Martin Codyre; Fernan Federici; Nathan A Tanner; Scott W Tighe; Randy True; Sarah B Ware; Anne L Wyllie; Evan E Afshin; Andres Bendesky; Connie B Chang; Richard Dela Rosa; Eran Elhaik; David Erickson; Andrew S Goldsborough; George Grills; Kathrin Hadasch; Andrew Hayden; Seong-Young Her; Julie A Karl; Chang Hee Kim; Alison J Kriegel; Thomas Kunstman; Zeph Landau; Kevin Land; Bradley W Langhorst; Ariel B Lindner; Benjamin E Mayer; Lee A McLaughlin; Matthew T McLaughlin; Jenny Molloy; Christopher Mozsary; Jerry L Nadler; Melinee D'Silva; David Ng; David H O'Connor; Jerry E Ongerth; Olayinka Osuolale; Ana Pinharanda; Dennis Plenker; Ravi Ranjan; Michael Rosbash; Assaf Rotem; Jacob Segarra; Stephan Schürer; Scott Sherrill-Mix; Helena Solo-Gabriele; Shaina To; Merly C Vogt; Albert D Yu; Christopher E Mason
Journal:  J Biomol Tech       Date:  2021-09

3.  Discrimination between right and wrong purine dNTPs by DNA polymerase I from Bacillus stearothermophilus.

Authors:  Michael Trostler; Alison Delier; Jeff Beckman; Milan Urban; Jennifer N Patro; Thomas E Spratt; Lorena S Beese; Robert D Kuchta
Journal:  Biochemistry       Date:  2009-06-02       Impact factor: 3.162

4.  Enhancement of Polymerase Activity of the Large Fragment in DNA Polymerase I from Geobacillus stearothermophilus by Site-Directed Mutagenesis at the Active Site.

Authors:  Yi Ma; Beilei Zhang; Meng Wang; Yanghui Ou; Jufang Wang; Shan Li
Journal:  Biomed Res Int       Date:  2016-11-17       Impact factor: 3.411

5.  Cellular reagents for diagnostics and synthetic biology.

Authors:  Sanchita Bhadra; Arti Pothukuchy; Raghav Shroff; Austin W Cole; Michelle Byrom; Jared W Ellefson; Jimmy D Gollihar; Andrew D Ellington
Journal:  PLoS One       Date:  2018-08-15       Impact factor: 3.240

6.  A Rapid, Highly Sensitive and Open-Access SARS-CoV-2 Detection Assay for Laboratory and Home Testing.

Authors:  Max J Kellner; James J Ross; Jakob Schnabl; Marcus P S Dekens; Martin Matl; Robert Heinen; Irina Grishkovskaya; Benedikt Bauer; Johannes Stadlmann; Luis Menéndez-Arias; Andrew D Straw; Robert Fritsche-Polanz; Marianna Traugott; Tamara Seitz; Alexander Zoufaly; Manuela Födinger; Christoph Wenisch; Johannes Zuber; Andrea Pauli; Julius Brennecke
Journal:  Front Mol Biosci       Date:  2022-04-01
  6 in total

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